Svendsen M B S, Bushnell P G, Christensen E A F, Steffensen J F
Marine Biological Section, Department of Biology, University of Copenhagen, Strandpromenaden 5, DK-3000 Helsingør, Denmark.
Department of Biological Sciences, Indiana University South Bend, South Bend, IN 46634, U.S.A.
J Fish Biol. 2016 Jan;88(1):51-64. doi: 10.1111/jfb.12851.
As intermittent-flow respirometry has become a common method for the determination of resting metabolism or standard metabolic rate (SMR), this study investigated how much of the variability seen in the experiments was due to measurement error. Experiments simulated different constant oxygen consumption rates (M˙O2 ) of a fish, by continuously injecting anoxic water into a respirometer, altering the injection rate to correct for the washout error. The effect of respirometer-to-fish volume ratio (RFR) on SMR measurement and variability was also investigated, using the simulated constant M˙O2 and the M˙O2 of seven roach Rutilus rutilus in respirometers of two different sizes. The results show that higher RFR increases measurement variability but does not change the mean SMR established using a double Gaussian fit. Further, the study demonstrates that the variation observed when determining oxygen consumption rates of fishes in systems with reasonable RFRs mainly comes from the animal, not from the measuring equipment.
由于间歇流呼吸测定法已成为测定静息代谢或标准代谢率（SMR）的常用方法，本研究调查了实验中观察到的变异性有多少是由测量误差引起的。实验通过向呼吸仪中连续注入缺氧水来模拟鱼的不同恒定耗氧率（M˙O2），改变注入速率以校正冲洗误差。还使用模拟的恒定M˙O2以及在两种不同尺寸呼吸仪中的七条拟鲤（Rutilus rutilus）的M˙O2，研究了呼吸仪与鱼的体积比（RFR）对SMR测量和变异性的影响。结果表明，较高的RFR会增加测量变异性，但不会改变使用双高斯拟合确定的平均SMR。此外，该研究表明，在具有合理RFR的系统中测定鱼类耗氧率时观察到的变化主要来自动物本身，而非测量设备。
