腰果过敏原Ana o 1、Ana o 2和Ana o 3的纯化与鉴定
Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3.
作者信息
Reitsma Marit, Bastiaan-Net Shanna, Sforza Stefano, van der Valk Johanna P M, van Gerth van Wijk Roy, Savelkoul Huub F J, de Jong Nicolette W, Wichers Harry J
机构信息
Department of Food Science, University of Parma , 43124 Parma, Italy.
Department of Internal Medicine, section Allergology, ErasmusMC , 3015 CE Rotterdam, The Netherlands.
出版信息
J Agric Food Chem. 2016 Feb 10;64(5):1191-201. doi: 10.1021/acs.jafc.5b04401. Epub 2016 Jan 29.
In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.
本研究描述了一种从腰果中快速简单地纯化三种已知过敏原（7S球蛋白Ana o 1、11S球蛋白Ana o 2和2S白蛋白Ana o 3）的方法。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）、蛋白质印迹法、糖蛋白染色和蛋白质鉴定对纯化的过敏原进行表征。纯化后的蛋白质仍能结合IgE，且不同患者血清池之间的IgE结合情况有所不同。发现Ana o 1是一种糖蛋白。对Ana o 3进行了更详细的研究，以鉴定其大小亚基，二者均表现出微异质性，并且已对Ana o 3进行了表位作图。
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