一种携带来自流感嗜血杆菌和大肠杆菌中pKM101的mucA和mucB基因的质粒。
A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli.
作者信息
Spikes D, Setlow J K
机构信息
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
出版信息
J Bacteriol. 1989 Oct;171(10):5753-5. doi: 10.1128/jb.171.10.5753-5755.1989.
Abstract
The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain. In H. influenzae, pMucAMucB caused a small but reproducible increase in survival after UV irradiation in wild-type cells and in a rec-1 mutant, but there was no increase in spontaneous mutation in the wild type or in the rec-1 mutant and no UV-induced mutation.
摘要
由流感嗜血杆菌载体pDM2构建的质粒pMucAMucB，以及由pBR322构建的类似质粒，提高了携带umu - 36突变的大肠杆菌AB1157在紫外线照射后的存活率，并且还在该大肠杆菌菌株中引起了紫外线诱导的突变。在流感嗜血杆菌中，pMucAMucB使野生型细胞和rec - 1突变体在紫外线照射后的存活率有小幅但可重复的提高，但野生型或rec - 1突变体的自发突变没有增加，也没有紫外线诱导的突变。
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