Setlow J K, Spikes D, Griffin K
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
J Bacteriol. 1988 Sep;170(9):3876-81. doi: 10.1128/jb.170.9.3876-3881.1988.
The rec-1 gene of Haemophilus influenzae was cloned into a shuttle vector that replicates in Escherichia coli as well as in H. influenzae. The plasmid, called pRec1, complemented the defects of a rec-1 mutant in repair of UV damage, transformation, and ability of prophage to be induced by UV radiation. Although UV resistance and recombination were caused by pRec1 in E. coli recA mutants, UV induction of lambda and UV mutagenesis were not. We suggest that the ability of the H. influenzae Rec-1 protein to cause cleavage of repressors but not the recombinase function differs from that of the E. coli RecA protein.
流感嗜血杆菌的rec-1基因被克隆到一种穿梭载体中,该载体可在大肠杆菌和流感嗜血杆菌中复制。这种名为pRec1的质粒弥补了rec-1突变体在紫外线损伤修复、转化以及原噬菌体被紫外线诱导能力方面的缺陷。尽管pRec1在大肠杆菌recA突变体中引起了紫外线抗性和重组,但并未引起λ噬菌体的紫外线诱导和紫外线诱变。我们认为,流感嗜血杆菌Rec-1蛋白引起阻遏物裂解的能力,而非重组酶功能,与大肠杆菌RecA蛋白不同。