流感嗜血杆菌rec-1基因的特性及其在大肠杆菌中的行为
Characterization of the rec-1 gene of Haemophilus influenzae and behavior of the gene in Escherichia coli.
作者信息
Setlow J K, Spikes D, Griffin K
机构信息
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
出版信息
J Bacteriol. 1988 Sep;170(9):3876-81. doi: 10.1128/jb.170.9.3876-3881.1988.
Abstract
The rec-1 gene of Haemophilus influenzae was cloned into a shuttle vector that replicates in Escherichia coli as well as in H. influenzae. The plasmid, called pRec1, complemented the defects of a rec-1 mutant in repair of UV damage, transformation, and ability of prophage to be induced by UV radiation. Although UV resistance and recombination were caused by pRec1 in E. coli recA mutants, UV induction of lambda and UV mutagenesis were not. We suggest that the ability of the H. influenzae Rec-1 protein to cause cleavage of repressors but not the recombinase function differs from that of the E. coli RecA protein.
摘要
流感嗜血杆菌的rec-1基因被克隆到一种穿梭载体中，该载体可在大肠杆菌和流感嗜血杆菌中复制。这种名为pRec1的质粒弥补了rec-1突变体在紫外线损伤修复、转化以及原噬菌体被紫外线诱导能力方面的缺陷。尽管pRec1在大肠杆菌recA突变体中引起了紫外线抗性和重组，但并未引起λ噬菌体的紫外线诱导和紫外线诱变。我们认为，流感嗜血杆菌Rec-1蛋白引起阻遏物裂解的能力，而非重组酶功能，与大肠杆菌RecA蛋白不同。
相似文献
1
Characterization of the rec-1 gene of Haemophilus influenzae and behavior of the gene in Escherichia coli.流感嗜血杆菌rec-1基因的特性及其在大肠杆菌中的行为
J Bacteriol. 1988 Sep;170(9):3876-81. doi: 10.1128/jb.170.9.3876-3881.1988.
2
Structural organization, nucleotide sequence, and regulation of the Haemophilus influenzae rec-1+ gene.流感嗜血杆菌rec - 1+基因的结构组织、核苷酸序列及调控
J Bacteriol. 1993 Nov;175(22):7269-81. doi: 10.1128/jb.175.22.7269-7281.1993.
3
Plasmid containing a DNA ligase gene from Haemophilus influenzae.含有来自流感嗜血杆菌的DNA连接酶基因的质粒。
J Bacteriol. 1984 May;158(2):730-2. doi: 10.1128/jb.158.2.730-732.1984.
4
A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli.一种携带来自流感嗜血杆菌和大肠杆菌中pKM101的mucA和mucB基因的质粒。
J Bacteriol. 1989 Oct;171(10):5753-5. doi: 10.1128/jb.171.10.5753-5755.1989.
5
Cloning and characterization of the Haemophilus influenzae mutB gene.流感嗜血杆菌mutB基因的克隆与特性分析
J Bacteriol. 1993 Aug;175(16):5265-7. doi: 10.1128/jb.175.16.5265-5267.1993.
6
Characterization of the Pseudomonas aeruginosa recA analog and its protein product: rec-102 is a mutant allele of the P. aeruginosa PAO recA gene.铜绿假单胞菌recA类似物及其蛋白质产物的表征：rec-102是铜绿假单胞菌PAO recA基因的一个突变等位基因。
J Bacteriol. 1987 Apr;169(4):1499-508. doi: 10.1128/jb.169.4.1499-1508.1987.
7
Two Haemophilus influenzae Rd genes that complement the recA-like mutation rec-1.两个可补充类recA突变rec-1的流感嗜血杆菌Rd基因。
J Bacteriol. 1989 May;171(5):2451-7. doi: 10.1128/jb.171.5.2451-2457.1989.
8
MucA protein affects spontaneous mutation in a localized region of Haemophilus influenzae.MucA蛋白影响流感嗜血杆菌局部区域的自发突变。
Mutat Res. 2001 Jul 1;478(1-2):75-81. doi: 10.1016/s0027-5107(01)00099-9.
9
Genes from plasmid pKM101 in Haemophilus influenzae: separation of functions of mucA and mucB.流感嗜血杆菌中质粒pKM101的基因：mucA和mucB功能的分离
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7753-6. doi: 10.1073/pnas.82.22.7753.
10
Properties of Haemophilus influenzae mutants that are slightly recombination deficient and carry a mutation in the rec-1 gene region.轻度重组缺陷且rec-1基因区域携带突变的流感嗜血杆菌突变体的特性。
J Bacteriol. 1980 Jun;142(3):829-35. doi: 10.1128/jb.142.3.829-835.1980.
引用本文的文献
1
Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila.嗜肺军团菌的自然转化独立于必需的肌动蛋白样MreB细胞骨架而发生。
Sci Rep. 2015 Nov 3;5:16033. doi: 10.1038/srep16033.
2
The OxyR regulon in nontypeable Haemophilus influenzae.不可分型流感嗜血杆菌中的OxyR调控子。
J Bacteriol. 2007 Feb;189(3):1004-12. doi: 10.1128/JB.01040-06. Epub 2006 Dec 1.
3
Structural organization, nucleotide sequence, and regulation of the Haemophilus influenzae rec-1+ gene.流感嗜血杆菌rec - 1+基因的结构组织、核苷酸序列及调控
J Bacteriol. 1993 Nov;175(22):7269-81. doi: 10.1128/jb.175.22.7269-7281.1993.
4
Cloning and characterization of the Haemophilus influenzae Rd rec-1+ gene.流感嗜血杆菌Rd rec-1+基因的克隆与特性分析
J Bacteriol. 1989 Aug;171(8):4395-401. doi: 10.1128/jb.171.8.4395-4401.1989.
5
Two Haemophilus influenzae Rd genes that complement the recA-like mutation rec-1.两个可补充类recA突变rec-1的流感嗜血杆菌Rd基因。
J Bacteriol. 1989 May;171(5):2451-7. doi: 10.1128/jb.171.5.2451-2457.1989.
6
A plasmid carrying mucA and mucB genes from pKM101 in Haemophilus influenzae and Escherichia coli.一种携带来自流感嗜血杆菌和大肠杆菌中pKM101的mucA和mucB基因的质粒。
J Bacteriol. 1989 Oct;171(10):5753-5. doi: 10.1128/jb.171.10.5753-5755.1989.
7
Regulation of the SOS response in Bacillus subtilis: evidence for a LexA repressor homolog.枯草芽孢杆菌中SOS反应的调控：LexA阻遏物同源物的证据。
J Bacteriol. 1991 Oct;173(20):6489-98. doi: 10.1128/jb.173.20.6489-6498.1991.
8
Molecular cloning of two linked loci that increase the transformability of transformation-deficient mutants of Haemophilus influenzae.增加流感嗜血杆菌转化缺陷型突变体转化能力的两个连锁基因座的分子克隆。
J Bacteriol. 1991 Aug;173(15):4675-82. doi: 10.1128/jb.173.15.4675-4682.1991.
9
Cloning, characterization, and DNA base sequence of the high-level streptomycin resistance gene strA1 of Haemophilus influenzae Rd.流感嗜血杆菌Rd的高水平链霉素抗性基因strA1的克隆、特性分析及DNA碱基序列
J Bacteriol. 1992 Sep;174(17):5604-8. doi: 10.1128/jb.174.17.5604-5608.1992.
10
rec-2-dependent phage recombination in Haemophilus influenzae.流感嗜血杆菌中rec-2依赖性噬菌体重组
J Bacteriol. 1992 Aug;174(15):4960-6. doi: 10.1128/jb.174.15.4960-4966.1992.
本文引用的文献
1
Dependence of Vegetative Recombination Among Haemophilus influenzae Bacteriophage on the Host Cell.B型流感嗜血杆菌噬菌体中营养重组对宿主细胞的依赖性
J Virol. 1969 Sep;4(3):240-3. doi: 10.1128/JVI.4.3.240-243.1969.
2
INFECTION OF TRANSFORMABLE CELLS OF HAEMOPHILUS INFLUENZAE BY BACTERIOPHAGE AND BACTERIOPHAGE DNA.流感嗜血杆菌可转化细胞被噬菌体及噬菌体DNA感染
Z Vererbungsl. 1963 Dec 30;94:336-48. doi: 10.1007/BF00897593.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
4
Transformation of Haemophilus influenzae by plasmid RSF0885.质粒RSF0885介导的流感嗜血杆菌转化
J Bacteriol. 1981 Dec;148(3):812-6. doi: 10.1128/jb.148.3.812-816.1981.
5
Transformation of Haemophilus influenzae by plasmid RSF0885 containing a cloned segment of chromosomal deoxyribonucleic acid.含有克隆的染色体脱氧核糖核酸片段的质粒RSF0885对流感嗜血杆菌的转化作用。
J Bacteriol. 1981 Dec;148(3):804-11. doi: 10.1128/jb.148.3.804-811.1981.
6
Sequences of the recA gene and protein.recA基因和蛋白质的序列。
Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5. doi: 10.1073/pnas.77.5.2611.
7
Identification of plasmid (pKM101)-coded proteins involved in mutagenesis and UV resistance.鉴定参与诱变和紫外线抗性的质粒（pKM101）编码蛋白。
Nature. 1982 Nov 18;300(5889):278-81. doi: 10.1038/300278a0.
8
Isolation and characterization of the recA gene of Rhizobium meliloti.苜蓿根瘤菌recA基因的分离与鉴定
J Bacteriol. 1983 Jul;155(1):311-6. doi: 10.1128/jb.155.1.311-316.1983.
9
A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli.一种用于流感嗜血杆菌和大肠杆菌的质粒克隆载体。
J Bacteriol. 1982 Sep;151(3):1605-7. doi: 10.1128/jb.151.3.1605-1607.1982.
10
Organization of the recA gene of Escherichia coli.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):313-7. doi: 10.1073/pnas.77.1.313.
Zotero 插件