Department of Surgery, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou, Zhejiang Province, China.
PLoS One. 2012;7(5):e37347. doi: 10.1371/journal.pone.0037347. Epub 2012 May 24.
To investigate the therapeutic effects of fluorouracil (5-Fu) and octreotide (Oct) continuous regional arterial infusion (CRAI,) alone or in combination, was administered in a canine model of severe acute pancreatitis (SAP).
The animals were divided into five groups; group A (Sham), group B (SAP), group C (SAP and 5-Fu), group D (SAP and Oct), and group E (SAP and 5-Fu + Oct). Levels of amylase, α-tumor necrosis factor (TNF-α), blood urea nitrogen (BUN), creatinine, thromboxane B2 and 6-keto- prostaglandin F1α were measured both before and after the induction of SAP. Pathologic examination of the pancreas and kidneys was performed after termination of the study.
Pathologic changes noted in the pancreas in SAP significantly improved following CRAI with either single or combined administration of 5-Fu and Oct, where combination therapy demonstrated the lowest injury score. All treatment groups had significantly lower levels of serum TNF-α and amylase activity (P<0.05), though only groups D and E had a lower BUN level as compared to group B. The plasma thromboxane B(2) level increased in SAP, but the ratio of thromboxane B(2)/6-keto- prostaglandin F(1α) decreased in the treatment groups, with the combination therapy (group E) demonstrating the lowest ratio as compared to the other 3 experimental groups (P<0.05).
The findings in the present study demonstrate an attenuation of SAP in a canine model following CRAI administration with 5-Fu or Oct, alone or in combination.
研究氟尿嘧啶（5-Fu）和奥曲肽（Oct）单独或联合持续区域性动脉灌注（CRAI）治疗重症急性胰腺炎（SAP）的疗效。
动物分为五组；A 组（假手术）、B 组（SAP）、C 组（SAP 和 5-Fu）、D 组（SAP 和 Oct）和 E 组（SAP 和 5-Fu + Oct）。在 SAP 诱导前后测定淀粉酶、α-肿瘤坏死因子（TNF-α）、血尿素氮（BUN）、肌酐、血栓素 B2 和 6-酮-前列腺素 F1α水平。研究结束后对胰腺和肾脏进行病理检查。
SAP 时胰腺的病理变化经 CRAI 后明显改善，无论是单独使用 5-Fu 和 Oct 还是联合使用，联合治疗显示出最低的损伤评分。所有治疗组的血清 TNF-α和淀粉酶活性均显著降低（P<0.05），但只有 D 组和 E 组的 BUN 水平低于 B 组。SAP 时血浆血栓素 B2 水平升高，但治疗组血栓素 B2/6-酮-前列腺素 F1α比值降低，联合治疗组（E 组）与其他 3 个实验组相比比值最低（P<0.05）。
本研究结果表明，在犬 SAP 模型中，CRAI 联合 5-Fu 或 Oct 单独或联合应用可减轻 SAP。
