Xiping Zhang, Hua Tian, Hanqing Chen, Li Chen, Binyan Yu, Jing Ma
Department of General Surgery, Hangzhou First People's Hospital, Hangzhou 310006, Zhejiang Province, China.
J Res Med Sci. 2009 Jan;14(1):19-27.
To investigate the effects of Baicalin and Octreotide on inflammatory mediators and pancreatic acinar cells apoptosis of rats with severe acute pancreatitis (SAP).
SD rats were randomly divided into sham operated group (I group), model control group (II group), Baicalin treated group (III group) and Octreotide treated group (IV group). Each group was also divided into subgroup of 3, 6 and 12 h (n = 15). The mortality rate, ascites/body weight ratio as well as the level of endotoxin, NO and ET-1 in blood were measured. The pathological severity score of pancreas, apoptotic indexes, and expression levels of Bax and Bcl-2 proteins in each group were investigated.
The survival rate of III and IV group has a significant difference compared with II group (P(12 h) < 0.05). The ascites volume, contents of inflammatory mediators in blood and pathological severity score of pancreas of III and IV group declined at different degrees compared to II group (P < 0.05, P < 0.01 or P < 0.001). Apoptotic index in III group was significantly higher than that in II group at 3 and 6 h (P(3, 6 h) < 0.05). Apoptotic index in IV group was significantly higher than that in II group at pancreatic tail at 6 h (P(6 h) < 0.05). Expression level of Bax in III group was significantly higher than that in II group (pancreatic head P(3 h,6 h) < 0.01, pancreatic tail P(3 h) < 0.001).
Compared with Octreotide in the treatment of SAP, the protective mechanisms of Baicalin include reducing the excessive inflammatory mediators' release, inducing the pancreatic acinar cells apoptosis.
探讨黄芩苷和奥曲肽对重症急性胰腺炎（SAP）大鼠炎症介质及胰腺腺泡细胞凋亡的影响。
将SD大鼠随机分为假手术组（I组）、模型对照组（II组）、黄芩苷治疗组（III组）和奥曲肽治疗组（IV组）。每组又分为3、6和12小时亚组（n = 15）。测定死亡率、腹水/体重比以及血液中内毒素、一氧化氮和内皮素-1水平。观察每组胰腺病理严重程度评分、凋亡指数以及Bax和Bcl-2蛋白表达水平。
III组和IV组的生存率与II组相比有显著差异（P(12 h) < 0.05）。与II组相比，III组和IV组的腹水量、血液中炎症介质含量及胰腺病理严重程度评分均有不同程度下降（P < 0.05，P < 0.01或P < 0.001）。III组在3和6小时时凋亡指数显著高于II组（P(3, 6 h) < 0.05）。IV组在6小时时胰腺尾部凋亡指数显著高于II组（P(6 h) < 0.05）。III组Bax表达水平显著高于II组（胰头P(3 h,6 h) < 0.01，胰尾P(3 h) < 0.001）。
与奥曲肽治疗SAP相比，黄芩苷的保护机制包括减少过度炎症介质释放，诱导胰腺腺泡细胞凋亡。
