Yang Yao, Liu Hong, Wang Xi, Chen Long
Department of Gynecology, Qingdao Municipal Hospital 1 Jiaozhou Road, Qingdao 266000, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18123-9. eCollection 2015.
Cervical cancer is one of the leading causes of cancer-related death in woman worldwide. In the present study, we investigated the role of microRNA 664 (miR-664) in regulating cancer migration and chemotherapy sensitivity in cervical cancer.
Quantitative real-time PCR (qPCR) was used to assess the mRNA levels of miR-664 in both cervical cancer cell lines and cancer tissues from human patients. Lentiviral vector containing miR-664 mimics (lv-miR-664) was used to upregulate endogenous miR-664 in cervical cancer HeLa cells. The effects of miR-664 up-regulation on cervical cancer cell migration and cisplatin sensitivity were assessed by MTT and Cisplatin assays. Furthermore, the effect of miR-664 up-regulation on E-Cadherin expression was examined by western blot. E-Cadherin was then silenced by siRNA to examine its effect on miR-664 regulation on cervical cancer cell.
MiR-664 was downregulated in both cervical cancer cell lines and cancer tissues in patients. In HeLa cells, lentivirus mediated miR-664 up-regulation reduced cancer cell migration and increased chemosensitivity to cisplatin. Western blot showed E-Cadherin was upregulated upon miR-664 overexpression in HeLa cells. Genetic silencing of E-Cadherin by siRNA reversed the inhibitory effect of miR-664 up-regulation on cervical cancer cell migration.
Our study demonstrated that miR-664 played an important role in regulating cervical cancer, possibly modulated by E-Cadherin.
宫颈癌是全球女性癌症相关死亡的主要原因之一。在本研究中，我们调查了微小RNA 664（miR - 664）在调节宫颈癌细胞迁移和化疗敏感性中的作用。
采用定量实时聚合酶链反应（qPCR）评估miR - 664在人宫颈癌细胞系和癌组织中的mRNA水平。使用含有miR - 664模拟物的慢病毒载体（lv - miR - 664）上调宫颈癌HeLa细胞中的内源性miR - 664。通过MTT和顺铂试验评估miR - 664上调对宫颈癌细胞迁移和顺铂敏感性的影响。此外，通过蛋白质免疫印迹法检测miR - 664上调对E - 钙黏蛋白表达的影响。然后用小干扰RNA（siRNA）沉默E - 钙黏蛋白，以检测其对miR - 664调节宫颈癌细胞的影响。
miR - 664在宫颈癌细胞系和患者癌组织中均下调。在HeLa细胞中，慢病毒介导的miR - 664上调减少了癌细胞迁移并增加了对顺铂的化疗敏感性。蛋白质免疫印迹法显示，HeLa细胞中miR - 664过表达后E - 钙黏蛋白上调。用siRNA对E - 钙黏蛋白进行基因沉默可逆转miR - 664上调对宫颈癌细胞迁移的抑制作用。
我们的研究表明，miR - 664在调节宫颈癌中起重要作用，可能受E - 钙黏蛋白调控。
