Pourrezaei Samira, Letafati Arash, Molaverdi Ghazale, Norouzi Mehdi, Mozhgani Sayed-Hamidreza
Department of Virology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran.
BMC Res Notes. 2025 Jan 21;18(1):28. doi: 10.1186/s13104-025-07084-8.
Adult T-cell leukemia/lymphoma (ATLL) is a type of blood cancer related to human T-cell lymphotropic virus type 1 (HTLV-1). The principal aim of this study was to investigate cellular processes related to innate immune response, intracellular protein transport, and translational initiation regulation in individuals afflicted with ATLL and Acute lymphoblastic leukemia (ALL). Whole blood samples and peripheral blood mononuclear cells were collected from 10 viral ATLL patients and 10 ALL subjects. Real-time quantitative PCR was then performed to quantify mRNA expression levels of SMC6, FANCM, EIF4H, WDR7, RAB3GAP2, and IFN α/β. The study revealed some distinctions between ATLL and ALL patients. Particularly, RAB3GAP2 level (P = 0.028) was found to be elevated in ATLL patients compared to ALL. Conversely, expression levels of IFN-β (P = 0.31), SMC6 (P = 0.68), WDR7 (P = 0.43), EIF4H (P = 0.38), and FANCM (P = 0.57) were diminished in ATLL patients in contrast to ALL. These proteins play a pivotal role in both translation and immune activation, suggesting a potential correlation between the observed disparities and the virus-mediated progression of cancer. However, it is worth noting that the expression differences in FANCM, EIF4H, SMC6, and WDR7 between ATLL and ALL were minimal. This proposes that the underlying molecular mechanisms governing ATLL and ALL may largely overlap concerning these cellular processes. However, considerable increased expression of RAB3GAP2 was observed in ATLL compared to ALL.
成人T细胞白血病/淋巴瘤（ATLL）是一种与1型人类嗜T细胞病毒（HTLV-1）相关的血癌。本研究的主要目的是调查ATLL患者和急性淋巴细胞白血病（ALL）患者中与先天免疫反应、细胞内蛋白质运输及翻译起始调控相关的细胞过程。从10名病毒相关性ATLL患者和10名ALL受试者中采集全血样本和外周血单核细胞。然后进行实时定量PCR以量化SMC6、FANCM、EIF4H、WDR7、RAB3GAP2和IFNα/β的mRNA表达水平。该研究揭示了ATLL患者和ALL患者之间的一些差异。特别是，与ALL患者相比，发现ATLL患者的RAB3GAP2水平升高（P = 0.028）。相反，与ALL相比，ATLL患者中IFN-β（P = 0.31）、SMC6（P = 0.68）、WDR7（P = 0.43）、EIF4H（P = 0.38）和FANCM（P = 0.57）的表达水平降低。这些蛋白质在翻译和免疫激活中都起着关键作用，这表明观察到的差异与病毒介导的癌症进展之间可能存在潜在关联。然而，值得注意的是，ATLL和ALL之间FANCM、EIF4H、SMC6和WDR7的表达差异很小。这表明在这些细胞过程方面，控制ATLL和ALL的潜在分子机制可能在很大程度上重叠。然而，与ALL相比，在ATLL中观察到RAB3GAP2的表达显著增加。
