Ai Fen, Chen Manhua, Li Wei, Yang Yang, Xu Guizhong, Gui Feng, Liu Zhenxing, Bai Xiangyan, Chen Zhen
Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
Department of Cardiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18311-8. eCollection 2015.
The traditional Chinese medicinal Danshen (Salvia miltiorrhiza), has long been used to treat cardiovascular diseases, however, the mechanism underlying its effects remain unclear. Here, this study would to investigate the effects of Danshen injection on myocardial infarction-induced cardiac damage.
Danshen was injected into mice models at low dose (3 g/kg per day) or at high dose (6 g/kg per day) after left anterior descending coronary artery (LAD) ligation. After 4 weeks, cardiac function was evaluated by echocardiography. Paraffin sections of the hearts were used for isolectin GS-IB4 staining. Protein and mRNA expression levels of HIF1α and VEGFA were evaluated by western blotting and real-time polymerase chain reaction (RT-PCR).
The hearts showed significantly impaired angiogenesis and slightly increase of HIF1α and VEGFA expression after LAD ligation. The angiogenesis defect and heart failure were partially rescued in Danshen treatment mice with great increase of HIF1α and VEGFA mRNA levels and protein expression.
These results illustrated that the protective effects of Danshen injection in responsive cardiac angiogenesis were at least in part due to increased HIF1α and VEGFA expression.
传统中药丹参长期以来被用于治疗心血管疾病，然而，其作用的潜在机制仍不清楚。在此，本研究旨在探讨丹参注射液对心肌梗死所致心脏损伤的影响。
在左冠状动脉前降支（LAD）结扎后，以低剂量（每天3克/千克）或高剂量（每天6克/千克）将丹参注射到小鼠模型中。4周后，通过超声心动图评估心脏功能。心脏的石蜡切片用于异凝集素GS-IB4染色。通过蛋白质印迹法和实时聚合酶链反应（RT-PCR）评估HIF1α和VEGFA的蛋白质和mRNA表达水平。
LAD结扎后，心脏显示出明显受损的血管生成，且HIF1α和VEGFA表达略有增加。在丹参治疗的小鼠中，血管生成缺陷和心力衰竭得到部分缓解，HIF1α和VEGFA的mRNA水平和蛋白质表达大幅增加。
这些结果表明，丹参注射液对心脏血管生成的保护作用至少部分归因于HIF1α和VEGFA表达的增加。
