Kim Jeong-Nam, Lee Jun-Young, Shin Kang-Jae, Gil Young-Chul, Koh Ki-Seok, Song Wu-Chul
Department of Biomedical Laboratory Science, Masan University, Masan, Korea.
Department of Anatomy, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul, Korea.
Anat Cell Biol. 2015 Dec;48(4):258-61. doi: 10.5115/acb.2015.48.4.258. Epub 2015 Dec 21.
The current model of compact bone is that of a system of Haversian (longitudinal) canals connected by Volkmann's (transverse) canals. Models based on either histology or microcomputed tomography do not accurately represent the morphologic detail and microstructure of this system, especially that of the canal networks and their spatial relationships. The aim of the present study was to demonstrate the morphologic pattern and network of the Haversian system and to compare endosteal and periosteal sides in rats using three-dimensional (3D) reconstruction. Ten Sprague-Dawley rats aged 8-10 weeks were used. The femurs were harvested from each rat and fixed, decalcified with 10% EDTA-2Na, serially sectioned at a thickness of 5 µm, and then stained with hematoxylin and eosin. The serial sections were reconstructed three-dimensionally using Reconstruct software. The Haversian canals in the endosteal region were found to be large, highly interconnected, irregular, and close to neighboring canals. In contrast, the canals in the periosteal region were straight and small. This combined application of 3D reconstruction and histology examinations to the Haversian system has confirmed its microstructure, showing a branched network pattern on the endosteal side but not on the periosteal side.
目前密质骨的模型是由哈弗斯（纵向）管系统通过福尔克曼（横向）管相连组成的。基于组织学或显微计算机断层扫描的模型不能准确呈现该系统的形态细节和微观结构，尤其是管网络及其空间关系的细节。本研究的目的是利用三维（3D）重建技术展示大鼠哈弗斯系统的形态模式和网络，并比较骨内膜和骨膜侧的情况。选用了10只8 - 10周龄的斯普拉格 - 道利大鼠。从每只大鼠身上取下股骨并进行固定，用10%乙二胺四乙酸二钠（EDTA - 2Na）脱钙，以5微米的厚度连续切片，然后用苏木精和伊红染色。使用Reconstruct软件对连续切片进行三维重建。发现骨内膜区域的哈弗斯管较大、高度相互连接、不规则且靠近相邻的管道。相比之下，骨膜区域的管道则是直的且较小。这种将3D重建和组织学检查联合应用于哈弗斯系统的方法证实了其微观结构，显示骨内膜侧呈现分支网络模式，而骨膜侧则没有。
