影响质粒拷贝数的大肠杆菌基因pcnB的克隆与特性分析
Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number.
作者信息
March J B, Colloms M D, Hart-Davis D, Oliver I R, Masters M
机构信息
Department of Molecular Biology, University of Edinburgh.
出版信息
Mol Microbiol. 1989 Jul;3(7):903-10. doi: 10.1111/j.1365-2958.1989.tb00239.x.
Abstract
A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.
摘要
一个影响ColE1相关质粒拷贝数的基因pcnB已被克隆，并定位在大肠杆菌染色体上panD和fhu之间3.6分钟处。该基因编码一种以前未描述过的48 kD蛋白质。几个独立分离出的表现出相同表型（拷贝数减少）的突变体已被证明是pcnB基因缺陷型。
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