Zidi Ines, Kharrat Najla, Abdelhedi Rania, Hassine Amna Ben, Laaribi Ahmed Baligh, Yahia Hamza Ben, Abdelmoula Nouha Bouayed, Abid Leila, Rebai Ahmed, Rizzo Roberta
Laboratory Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Centre de Biotechnologie de Sfax, Molecular and Cellular Screening Process, route Sidi Mansour, BP1177, 3018 Sfax, Tunisia.
Hum Immunol. 2016 Apr;77(4):325-9. doi: 10.1016/j.humimm.2016.01.008. Epub 2016 Jan 11.
Several evidences suggest the association between the evolution of coronary artery disease (CAD) and the development of coronary syndrome that is often associated with disrupted plaque and partial or complete thrombosis of the related artery. Because of the inflammatory nature of CAD, we investigated the human leukocyte antigen (HLA)-G, HLA-E, and HLA-F genetic polymorphisms within CAD patients and evaluated their potential association with this disease in Tunisian population.
Different polymorphisms in HLA-G (14-bp Insertion/Deletion, +3142C/G), HLA-E (HLA-E01:01/01:03 A/G), HLA-F (HLA-F01:02 T/C, 01:03 C/T, 01:04 A/C) genes were typed using different laboratory techniques in a cohort of 89 CAD patients and 84 controls.
A significant association was reported between the HLA-G +3142 G allele (OR=1.64, 95% CI=1.05-2.56, p=0.02) and increased risk of CAD. No association was found for the other studied polymorphisms. When we considered the haplotypes, we found TDELCA and TDELGG haplotypes associated to CAD with p=0.008 and p=0.030, respectively, suggesting the potential interaction between HLA-G and HLA-E genes.
Our findings indicated that the HLA-G +3142C/G polymorphism and TDELCA and TDELGG haplotypes can harbour a reliable diagnosis value for the risk of CAD development suggesting that HLA-G, -E and -F molecules might be involved in the pathogenesis of the disease. However, further studies are necessary to confirm our results.
多项证据表明冠状动脉疾病（CAD）的进展与冠状动脉综合征的发生之间存在关联，冠状动脉综合征常与斑块破裂以及相关动脉的部分或完全血栓形成有关。鉴于CAD的炎症性质，我们研究了CAD患者体内的人类白细胞抗原（HLA）-G、HLA-E和HLA-F基因多态性，并评估了它们与突尼斯人群中该疾病的潜在关联。
采用不同实验室技术对89例CAD患者和84例对照组成的队列中的HLA-G（14-bp插入/缺失，+3142C/G）、HLA-E（HLA-E01:01/01:03 A/G）、HLA-F（HLA-F01:02 T/C、01:03 C/T、01:04 A/C）基因的不同多态性进行分型。
据报告，HLA-G +3142 G等位基因与CAD风险增加之间存在显著关联（OR=1.64，95%CI=1.05-2.56，p=0.02）。其他研究的多态性未发现关联。当我们考虑单倍型时，发现TDELCA和TDELGG单倍型与CAD相关，p值分别为0.008和0.030，提示HLA-G和HLA-E基因之间可能存在相互作用。
我们的研究结果表明，HLA-G +3142C/G多态性以及TDELCA和TDELGG单倍型可能对CAD发生风险具有可靠的诊断价值，提示HLA-G、-E和-F分子可能参与了该疾病的发病机制。然而，需要进一步研究来证实我们的结果。
