a Clinical Biology Department , Salah Azaiz Institute , Tunis , Tunisia.
b Jean Dausset Laboratory and INSERM, U1160 , Saint Louis Hospital , Paris , France.
Immunol Invest. 2019 Jul;48(5):521-532. doi: 10.1080/08820139.2019.1569043. Epub 2019 Apr 4.
Human leukocyte antigens G and E (HLA-G and HLA-E) are nonclassical major histocompatibility complex (MHC) class I molecules. These molecules play an important role in immune surveillance by inhibiting natural killer and cytotoxic T cells responsible for immune escape. The expression of HLA-G and HLA-E has been associated with several diseases including tumor. The main objective of the study is to evaluate the impact of three 3'UTR potential polymorphisms: +3187 A > G (rs9380142), +3142 G > C (rs1063320), +2960 14-base pair (bp) Insertion/Deletion (Ins/Del) (rs66554220), and the HLA-E*01:01/01:03 A > G (rs1264457) polymorphism in Tunisian breast cancer population. A total of 355 patients and 381 controls were genotyping for and polymorphisms using a Taq Man assay. +3142 allele and +3142 genotype were significantly associated with increased risk of breast cancer (p = 0.00002; OR = 1.58; 95% CI = 27-1.97) (49% versus 35%; p = 0.0001; OR = 1.79; 95% CI = 1.32-2.44). In addition, allele and the homozygous state for genotype confer a risk for breast cancer (52% versus 45%, p = 0.006; OR = 1.33, 95% CI = 1.08-1.64) (28% versus 22%, p = 0.039; OR = 1.43, 95% CI = 0.90-2.25). However, no statistical significant differences were reported for + 3187 A > G and variations and breast cancer in a Tunisian population. The found results indicate that HLA-G may play an important role in the breast cancer.
人类白细胞抗原 G 和 E（HLA-G 和 HLA-E）是非经典主要组织相容性复合体（MHC）I 类分子。这些分子通过抑制负责免疫逃逸的自然杀伤细胞和细胞毒性 T 细胞，在免疫监视中发挥重要作用。HLA-G 和 HLA-E 的表达与多种疾病有关，包括肿瘤。本研究的主要目的是评估三个 3'UTR 潜在多态性：+3187 A > G（rs9380142）、+3142 G > C（rs1063320）、+2960 14 碱基对（bp）插入/缺失（Ins/Del）（rs66554220）以及 HLA-E*01:01/01:03 A > G（rs1264457）多态性对突尼斯乳腺癌人群的影响。使用 Taq Man 测定法对 355 名患者和 381 名对照进行了 和 多态性的基因分型。+3142 等位基因和 +3142 基因型与乳腺癌风险增加显著相关（p = 0.00002；OR = 1.58；95%CI = 27-1.97）（49%比 35%；p = 0.0001；OR = 1.79；95%CI = 1.32-2.44）。此外， 等位基因和 基因型纯合状态使乳腺癌的风险增加（52%比 45%，p = 0.006；OR = 1.33，95%CI = 1.08-1.64）（28%比 22%，p = 0.039；OR = 1.43，95%CI = 0.90-2.25）。然而，在突尼斯人群中，未发现 HLA-G 可能在乳腺癌中发挥重要作用。
