Department of Chemistry, Jinan University, Guangzhou 510632, People's Republic of China.
Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Department of Environmental Engineering, Jinan University, Guangzhou 510632, People's Republic of China.
J Hazard Mater. 2016 Apr 15;307:154-62. doi: 10.1016/j.jhazmat.2015.12.069. Epub 2016 Jan 6.
Membrane concentrates of landfill leachates contain organic and inorganic contaminants that could be highly toxic and carcinogenic. In this paper, the genotoxicity of membrane concentrates before and after Fenton and UV-Fenton reagent was assessed. The cytotoxicity and genotoxicity was determined by using the methods of methyltetrazolium (MTT), cytokinesis-block micronucleus (CBMN) and comet assay in human hepatoma cells. MTT assay showed a cytotoxicity of 75% after 24h of exposure to the highest tested concentration of untreated concentrates, and no cytotoxocity for UV-Fenton and Fenton treated concentrates. Both CBMN and comet assays showed increased levels of genotoxicity in cells exposed to untreated concentrates, compared to those occurred in cells exposed to UV-Fenton and Fenton reagent treated concentrates. There was no significant difference between negative control and UV-Fenton treated concentrates for micronucleus and comet assay parameters. UV-Fenton and Fenton treatment, especially the former, were effective methods for degradation of bisphenol A and nonylphenol in concentrates. These findings showed UV-Fenton and Fenton reaction were effective methods for treatment of such complex concentrates, UV-Fenton reagent provided toxicological safety of the treated effluent, and the genotoxicity assays were found to be feasible tools for assessment of toxicity risks of complex concentrates.
垃圾渗滤液膜浓缩物含有有机和无机污染物，这些污染物可能具有高度毒性和致癌性。本文评估了芬顿试剂和 UV-Fenton 试剂处理前后膜浓缩物的遗传毒性。采用噻唑蓝（MTT）法、胞质阻断微核（CBMN）法和彗星试验法，测定了人肝癌细胞的细胞毒性和遗传毒性。MTT 试验结果表明，未经处理的浓缩物最高测试浓度暴露 24h 后，细胞的细胞毒性为 75%，而 UV-Fenton 和 Fenton 处理的浓缩物则没有细胞毒性。CBMN 和彗星试验均表明，与 UV-Fenton 和 Fenton 处理的浓缩物相比，未经处理的浓缩物暴露的细胞中遗传毒性水平升高。微核和彗星试验参数的阴性对照与 UV-Fenton 处理的浓缩物之间无显著差异。UV-Fenton 和 Fenton 处理，尤其是前者，是浓缩物中双酚 A 和壬基酚降解的有效方法。这些发现表明，UV-Fenton 和 Fenton 反应是处理此类复杂浓缩物的有效方法，UV-Fenton 试剂为处理后的废水提供了毒理学安全性，并且遗传毒性试验被证明是评估复杂浓缩物毒性风险的可行工具。
