Hou Changcheng, Lu Gang, Zhao Ling, Yin Pinghe, Zhu Lingfei
School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.
School of Environment, Guangzhou Key Laboratory of Environmental Exposure and Health, Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.
Chemosphere. 2017 Aug;180:192-200. doi: 10.1016/j.chemosphere.2017.04.033. Epub 2017 Apr 8.
Membrane concentrates (MCs) are generated when membranes are used to concentrate landfill leachate. It contains high concentrations of inorganic and organic environmental pollutants, which are highly toxic and carcinogenic. In this paper, the proliferation effect (PE) from MC before and after treatment with the UV-Fenton process was assessed using the human breast carcinoma cell line MCF-7. The highest value of 116% was found at 5% (v/v) concentration after a 10 min reaction. Phthalic acid esters (PAEs) play an important role in the MC estrogenicity. Estrogen simulation solutions (ESS) of PAEs were prepared to simulate the changes in estrogenic active substances during the UV-Fenton process. The ESS degradation conformed to the first-order kinetics model. The estrogenicity decreased after an initial increase until it acted in a non-estrogenic manner. Convincingly, the intermediates were determined by GC/MS, and the estrogenicity was assessed during the degradation process. The estrogenicity was highly related to the generation of intermediates and the PAE concentration. The results provide guidance for UV-Fenton application in MC estrogenicity reduction.
当使用膜对垃圾渗滤液进行浓缩时会产生膜浓缩液（MCs）。它含有高浓度的无机和有机环境污染物，这些污染物具有高毒性和致癌性。本文使用人乳腺癌细胞系MCF-7评估了紫外线-芬顿法处理前后MC的增殖效应（PE）。在10分钟反应后，5%（v/v）浓度下发现最高值为116%。邻苯二甲酸酯（PAEs）在MC的雌激素活性中起重要作用。制备了PAEs的雌激素模拟溶液（ESS）以模拟紫外线-芬顿过程中雌激素活性物质的变化。ESS的降解符合一级动力学模型。雌激素活性在最初增加后下降，直至以非雌激素方式起作用。令人信服的是，通过气相色谱/质谱联用仪（GC/MS）确定了中间体，并在降解过程中评估了雌激素活性。雌激素活性与中间体的生成和PAE浓度高度相关。研究结果为紫外线-芬顿法在降低MC雌激素活性方面的应用提供了指导。
