De Vuyst Luc, Vincent Pascal, Makras Eleftherios, Leroy Frédéric, Pot Bruno
Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bio-engineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium.
Equipe Mixte Inserm E9919-Université JE 2225-Institut de Biologie de Lille, rue du Pr Calmette 1, B.P. 245, 59021, Lille, France.
Probiotics Antimicrob Proteins. 2010 Mar;2(1):26-36. doi: 10.1007/s12602-009-9029-4.
Helicobacter pylori inhibition by probiotic lactobacilli has been observed in vitro and in vivo. Carefully selected probiotic Lactobacillus strains could therefore play an important role in the treatment of H. pylori infection and eradication. However, the underlying mechanism for this inhibition is not clear. The aim of this study was to examine if peptide extracts, containing bacteriocins or other antibacterial peptides, from six Lactobacillus cultures (Lactobacillus acidophilus La1, Lactobacillus amylovorus DCE 471, Lactobacillus casei YIT 9029, Lactobacillus gasseri K7, Lactobacillus johnsonii La1, and Lactobacillus rhamnosus GG) contribute to the inhibition of H. pylori. Peptide extracts from cultures of Lact. amylovorus DCE 471 and Lact. johnsonii La1 were most active, reducing the viability of H. pylori ATCC 43504 with more than 2 log units within 4 h of incubation (P < 0.001). The four other extracts were less or not active. When six clinical isolates of H. pylori were tested for their susceptibility towards five inhibitory peptide extracts, similar observations were made. Again, the peptide extracts from Lact. amylovorus DCE 471 and Lact. johnsonii La1 were the most inhibitory, while the three other extracts resulted in a much lower inhibition of H. pylori. Protease-treated extracts were inactive towards H. pylori, confirming the proteinaceous nature of the inhibitory substance.
在体外和体内均观察到益生菌乳酸菌对幽门螺杆菌的抑制作用。因此，精心挑选的益生菌乳酸杆菌菌株可能在幽门螺杆菌感染的治疗和根除中发挥重要作用。然而，这种抑制作用的潜在机制尚不清楚。本研究的目的是检验来自六种乳酸杆菌培养物（嗜酸乳杆菌La1、解淀粉乳杆菌DCE 471、干酪乳杆菌YIT 9029、加氏乳杆菌K7、约氏乳杆菌La1和鼠李糖乳杆菌GG）的含有细菌素或其他抗菌肽的肽提取物是否有助于抑制幽门螺杆菌。解淀粉乳杆菌DCE 471和约氏乳杆菌La1培养物的肽提取物活性最高，在孵育4小时内使幽门螺杆菌ATCC 43504的活力降低超过2个对数单位（P < 0.001）。其他四种提取物活性较低或无活性。当检测六种幽门螺杆菌临床分离株对五种抑制性肽提取物的敏感性时，观察到类似结果。同样，解淀粉乳杆菌DCE 471和约氏乳杆菌La1的肽提取物抑制作用最强，而其他三种提取物对幽门螺杆菌的抑制作用则低得多。经蛋白酶处理的提取物对幽门螺杆菌无活性，证实了抑制物质的蛋白质性质。
