Yeddes Nizar, Chérif Jamila K, Guyot Sylvain, Sotin Hélène, Ayadi Malika T
Laboratory Applications of Chemical Resources, Natural Substances and the Environment (LACReSNE), Faculty of Sciences of Bizerte, University of Carthage, 7021 Zarzouna-Bizerte, Tunisia.
Tunis Preparatory Institute for Engineering Studies (IPEIT), Montfleury, University of Tunis, 1008 Tunis, Tunisia.
Antioxidants (Basel). 2013 Apr 19;2(2):37-51. doi: 10.3390/antiox2020037.
The antioxidant activity and the chemical composition of methanol extracts from peel and pulp belonging to two species of Tunisian prickly pears Opuntia ficus indica (spiny and thornless forms) and Opuntia stricta have been studied. The antioxidant capacity was measured by DPPH radical scavenging activity. The total phenolic compound (TPC) and the total flavonoid content were determined by the Folin-Ciocalteu method and colorimetric method, respectively. The phenolic compounds were identified and quantified by high-performance liquid chromatography (HPLC) coupled with an electrospray ionization mass spectrometry (ESI-MS). The results showed that O. stricta fruits present the best antioxidant activities than the two forms of O. ficus indica, while the TPC was more important in O. ficus indica than in the O. stricta fruits. The peels have higher flavonoids than pulp, and the thornless variety has more flavonoid than the spiny. The RP-HPLC and ESI-MS analysis detected two classes of phenolic compounds and betalain pigments. Isorhamnetin derivatives are the dominant flavonol glycoside identified in O. ficus indica (spiny: 65.25 μg·g(-1); thornless: 77.03 μg·g(-1)) and O. stricta peels (19.22 μg·g(-1)).
对突尼斯两种仙人掌果（印度仙人掌（有刺和无刺形态）和直立仙人掌）果皮和果肉的甲醇提取物的抗氧化活性及化学成分进行了研究。通过DPPH自由基清除活性测定抗氧化能力。分别采用福林-酚法和比色法测定总酚化合物（TPC）和总黄酮含量。通过高效液相色谱（HPLC）结合电喷雾电离质谱（ESI-MS）对酚类化合物进行鉴定和定量。结果表明，直立仙人掌果实的抗氧化活性比印度仙人掌的两种形态都好，而印度仙人掌果实中的TPC比直立仙人掌果实中的更丰富。果皮中的黄酮类化合物比果肉中的多，无刺品种的黄酮类化合物比有刺品种的多。反相高效液相色谱（RP-HPLC）和电喷雾电离质谱（ESI-MS）分析检测到两类酚类化合物和甜菜色素。异鼠李素衍生物是在印度仙人掌（有刺：65.25μg·g⁻¹；无刺：77.03μg·g⁻¹）和直立仙人掌果皮（19.22μg·g⁻¹）中鉴定出的主要黄酮醇糖苷。
Zotero 插件
