Bubba L, Pellegrinelli L, Pariani E, Primache V, Amendola A, Binda S
Department of Biomedical Sciences for Health, University of Milan, Italy.
J Prev Med Hyg. 2015 Aug 5;56(2):E57-60.
Enterovirus (EV) and parechovirus (PeV) can either infect humans asymptomatically or can cause gastroenteritis, respiratory symptoms and, sometimes, severe disease. As the number of newly identified EV and PeV genotypes keeps increasing, diagnostic methods need to be updated. To this end, we described a novel multiplex one-step real-time RT-PCR to detect EV and human PeV (HPeV) simultaneously in fecal samples collected from children with rotavirus group A (RV-A)-related gastroenteritis.
The specificity and sensitivity of the EV/HPeV realtime RT-PCR were evaluated with two 2011 Quality Control for Molecular Diagnostics (QCMD) panels for EV and HPeV detection. RNA was extracted from 111 RV-A-positive fecal samples collected from children up to 5 years of age who had been hospitalized for gastroenteritis from September 2010 to August 2011.
The EV/HPeV real-time RT-PCR showed a 100% sensitivity and specificity for EV and 91% and 91.7% for HPeV, respectively. Of the 111 RV-A-positive stool specimens, 28 (25.2%) were EV-positive and 7 (6.3%) were HPeV-positive. No clinical differences between children with single or double infections were observed.
In our study, the frequency of EV and HPeV infections was surprisingly high, thus underlining the importance of including EV and HPeV detection in diagnostic panels. The multiplex real-time RT-PCR presented in this paper can therefore be a useful method in a diagnostic setting.
肠道病毒（EV）和细小病毒（PeV）感染人类后，既可能无症状，也可能引发肠胃炎、呼吸道症状，有时还会导致严重疾病。随着新发现的EV和PeV基因型数量不断增加，诊断方法需要更新。为此，我们描述了一种新型多重一步实时逆转录聚合酶链反应（RT-PCR），用于同时检测从感染A组轮状病毒（RV-A）相关肠胃炎儿童收集的粪便样本中的EV和人细小病毒（HPeV）。
使用两个2011年用于EV和HPeV检测的分子诊断质量控制（QCMD）面板评估EV/HPeV实时RT-PCR的特异性和敏感性。从2010年9月至2011年8月因肠胃炎住院的5岁以下儿童收集的111份RV-A阳性粪便样本中提取RNA。
EV/HPeV实时RT-PCR对EV的敏感性和特异性均为100%，对HPeV的敏感性和特异性分别为91%和91.7%。在111份RV-A阳性粪便标本中，28份（25.2%）为EV阳性，7份（6.3%）为HPeV阳性。未观察到单重或双重感染儿童之间的临床差异。
在我们的研究中，EV和HPeV感染的频率出奇地高，这凸显了在诊断面板中纳入EV和HPeV检测的重要性。因此，本文介绍的多重实时RT-PCR可成为诊断环境中的一种有用方法。
