Haertlein Michael, Moulin Martine, Devos Juliette M, Laux Valerie, Dunne Orla, Forsyth V Trevor
Life Sciences Group, Institut Laue-Langevin, Grenoble Cedex 9, France.
Life Sciences Group, Institut Laue-Langevin, Grenoble Cedex 9, France; Department of Structural and Molecular Biology, University College London, London, United Kingdom.
Methods Enzymol. 2016;566:113-57. doi: 10.1016/bs.mie.2015.11.001. Epub 2015 Dec 22.
Neutron scattering studies provide important information in structural biology that is not accessible using other approaches. The uniqueness of the technique, and its complementarity with X-ray scattering, is greatest when full use is made of deuterium labeling. The ability to produce tailor-made deuterium-labeled biological macromolecules allows neutron studies involving solution scattering, crystallography, reflection, and dynamics to be optimized in a manner that has major impact on the scope, quality, and throughput of work in these areas. Deuteration facilities have now been developed at many neutron centres throughout the world; these are having a crucial effect on neutron studies in the life sciences and on biologically related studies in soft matter. This chapter describes methods that have been developed for the efficient production of deuterium-labeled samples for a wide range of neutron scattering applications. Examples are given that illustrate the use of these samples for each of the main techniques. Perspectives for biological deuterium labeling are discussed in relation to developments at current facilities and those that are planned in the future.
中子散射研究在结构生物学中提供了重要信息，而这些信息是使用其他方法无法获得的。当充分利用氘标记时，该技术的独特性及其与X射线散射的互补性最为显著。生产定制的氘标记生物大分子的能力，使得涉及溶液散射、晶体学、反射和动力学的中子研究能够以一种对这些领域工作的范围、质量和通量产生重大影响的方式进行优化。现在，世界各地的许多中子中心都已开发出氘化设施；这些设施对生命科学中的中子研究以及软物质中与生物学相关的研究产生了至关重要的影响。本章介绍了为广泛的中子散射应用高效生产氘标记样品而开发的方法。给出了一些示例，说明了这些样品在每种主要技术中的应用。结合当前设施的发展以及未来计划中的发展，讨论了生物氘标记的前景。
