Ewald S J
Department of Microbiology, Montana State University, Bozeman 59717.
Alcohol Clin Exp Res. 1989 Aug;13(4):485-9. doi: 10.1111/j.1530-0277.1989.tb00362.x.
There are reports that fetal alcohol syndrome (FAS) is associated with immune deficiency or DiGeorge syndrome. To investigate the effect of prenatal alcohol exposure on the immune system, we used a mouse model of FAS in which C57BL/6J female mice were fed a complete liquid diet containing 25% ethanol-derived calories (EDC) from gestational day (g.d.) 1 to 18. Thymus cell numbers were markedly reduced in 18-day fetuses exposed to ethanol. Thymocytes from fetuses from the 25% EDC diet group and from pair-fed and ad-libitum control diet groups were compared by flow cytometry for expression of T cell differentiation antigens. The proportions of L3T4- and Lyt-2 positive thymus cells were significantly reduced in alcohol-exposed fetuses compared to controls; however, the number of Thy-1-positive cells did not differ among any of the groups. Six-day old neonates exposed prenatally to ethanol from g.d. 1 to 13 had thymus and spleen T cell populations similar to those of controls in almost all cases, indicating a "catch-up" of T cell numbers in most animals. Spleen T cell function, assessed by response to Concanavalin A (Con A), or Con A plus T cell growth factors, was somewhat depressed in ethanol-exposed 6-day pups.
有报道称胎儿酒精综合征（FAS）与免疫缺陷或迪格奥尔格综合征有关。为了研究产前酒精暴露对免疫系统的影响，我们使用了一种FAS小鼠模型，其中C57BL/6J雌性小鼠从妊娠第1天（g.d.）到第18天喂食含25%乙醇衍生热量（EDC）的完全液体饮食。暴露于乙醇的18天胎儿的胸腺细胞数量明显减少。通过流式细胞术比较了25% EDC饮食组、配对喂食组和随意进食对照组胎儿的胸腺细胞中T细胞分化抗原的表达。与对照组相比，酒精暴露胎儿中L3T4和Lyt-2阳性胸腺细胞的比例显著降低；然而，Thy-1阳性细胞的数量在任何组之间均无差异。从妊娠第1天到第13天产前暴露于乙醇的6日龄新生儿，在几乎所有情况下，其胸腺和脾脏T细胞群体与对照组相似，这表明大多数动物的T细胞数量出现了“追赶”现象。通过对刀豆球蛋白A（Con A）或Con A加T细胞生长因子的反应评估，暴露于乙醇的6日龄幼崽的脾脏T细胞功能略有下降。
