含牙囊肿、牙源性角化囊肿和根尖囊肿中肥大细胞及小血管的免疫组织化学评估
Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst.
作者信息
Kouhsoltani Maryam, Moradzadeh Khiavi Monir, Jamali Golshan, Farnia Samira
机构信息
Dental and Periodontal Research Center and Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Oral and Maxillofacial Pathology, Dental Faculty, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Adv Pharm Bull. 2015 Dec;5(Suppl 1):637-41. doi: 10.15171/apb.2015.086. Epub 2015 Dec 31.
PURPOSE
The aim of this study was to verify the density of mast cells (MCs) and microvessels in odontogenic cysts. Furthermore, the correlation between MCs and microvessels was evaluated to assess the contribution of MCs to angiogenesis and growth of odontogenic cysts. This approach may be a basis for the development of future pharmaceuticals addressed to MCs performance to manage odontogenic cysts. To our knowledge, no study investigating the correlation between MCs and microvessels has been performed to date.
METHODS
60 cases of odontogenic cysts consisting of 20 radicular cysts (RCs), 20 odontogenic keratocysts (OKCs) and 20 dentigerous cysts (DCs) were included in this study. Five high power fields in superficial connective tissue and five high power fields in deep connective tissue were counted for each sample. Moreover, a total mean of ten fields was calculated.
RESULTS
RC showed the highest mean numbers of MCs and microvessels (p<0.05). The subepithelial zones of all cysts contained more MCs and microvessels compared to the deeper zones. A statistically significant correlation between the numbers of MCs and microvessels was not observed (r=0.00, p=0.49).
CONCLUSION
Although the number of MCs was not significantly associated with microvessels, these cells may be related to the growth of odontogenic lesions, particularly RCs. Further studies on the in vivo functions of MCs will make the concept more clear.
目的
本研究旨在验证牙源性囊肿中肥大细胞（MCs）和微血管的密度。此外，评估MCs与微血管之间的相关性，以评估MCs对牙源性囊肿血管生成和生长的作用。这种方法可能是未来开发针对MCs功能以治疗牙源性囊肿的药物的基础。据我们所知，迄今为止尚未有研究调查MCs与微血管之间的相关性。
方法
本研究纳入60例牙源性囊肿，包括20例根端囊肿（RCs）、20例牙源性角化囊肿（OKCs）和20例含牙囊肿（DCs）。对每个样本在浅表结缔组织中计数5个高倍视野，在深部结缔组织中计数5个高倍视野。此外，计算10个视野的总平均值。
结果
RC显示MCs和微血管的平均数量最高（p<0.05）。与较深区域相比，所有囊肿的上皮下区域含有更多的MCs和微血管。未观察到MCs数量与微血管数量之间存在统计学显著相关性（r = 0.00，p = 0.49）。
结论
尽管MCs的数量与微血管无显著相关性，但这些细胞可能与牙源性病变尤其是RCs的生长有关。对MCs体内功能的进一步研究将使这一概念更加清晰。
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