Freeman A E, Lake R S, Igel H J, Gernand L, Pezzutti M R, Malone J M, Mark C, Benedict W F
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2451-5. doi: 10.1073/pnas.74.6.2451.
Cultured epithelial cells from human skin generally had 3- to 30-fold more hydrocarbon-metabolizing activity than fibroblasts from skin of the same donor. This activity was constant for up to 55 days in primary culture but was lost rapidly upon physical subdivision of the cultures. Treatment of primary mixed fibroblasts and epithelial cell cultures with methylcholanthrene, but not phenanthrene, led to development of actively growing fibroblastic cultures with many heteroploid cells. Unique marker chromosomes, stable over a number of cell population doublings, were identified in several of the heteroploid cell strains. Pure cultures of fibroblasts from the same donors did not undergo heteroploid conversion in response to methylcholanthrene. Spontaneously occurring heteroploidy in logarithmic phase human fibroblasts is a rare event; thus, heteroploid conversion may be a useful marker for chemical transformation of human cells. Because methylcholanthrene seems to have little transforming effect on human skin fibroblasts, human skin epithelial cells, because of their hydrocarbon-metabolizing activity, may serve to convert methylcholanthrene from a distal to an ultimate carcinogenic form.
来自人类皮肤的培养上皮细胞通常比来自同一供体皮肤的成纤维细胞具有多3至30倍的碳氢化合物代谢活性。这种活性在原代培养中长达55天保持恒定，但在培养物进行物理传代时会迅速丧失。用甲基胆蒽而非菲处理原代混合成纤维细胞和上皮细胞培养物，会导致形成具有许多异倍体细胞的活跃生长的成纤维细胞培养物。在几个异倍体细胞株中鉴定出了在多个细胞群体倍增过程中稳定的独特标记染色体。来自相同供体的成纤维细胞纯培养物在甲基胆蒽作用下不会发生异倍体转化。对数期人类成纤维细胞中自发出现的异倍体是一种罕见事件；因此，异倍体转化可能是人类细胞化学转化的一个有用标记。由于甲基胆蒽似乎对人类皮肤成纤维细胞几乎没有转化作用，而人类皮肤上皮细胞因其碳氢化合物代谢活性，可能有助于将甲基胆蒽从一种远端致癌形式转化为最终致癌形式。
