Felix J S, Sun T T, Littlefield J W
In Vitro. 1980 Oct;16(10):866-74. doi: 10.1007/BF02619424.
Epithelial cells can be cultured from the urine of newborn infants, providing a simple, noninvasive biopsy method. We established such cultures by standard techniques from 44% of uncontaminated specimens obtained from newborn infants up to 1 week of age. There was an average of three colonies per milliliter of urine. Many cultures accomplished 15 to 25 population doublings in as many as five subcultures and yielded total potential culture sizes of 10(4) to 6 x 10(8) cells. Plating efficiency was high at each passage. The cultures displayed two morphologically distinct epithelial cell types. Immunofluorescent staining of keratin fibers in most of these cells further identified them as epithelial.
上皮细胞可从新生儿尿液中培养获得，这提供了一种简单、无创的活检方法。我们采用标准技术，从年龄在1周以内的新生儿获取的44%未受污染的标本中成功建立了此类培养物。每毫升尿液平均有三个菌落。许多培养物在多达五次传代培养中实现了15至25次群体倍增，产生的总潜在培养细胞数量为10⁴至6×10⁸个。每次传代时的接种效率都很高。培养物显示出两种形态上不同的上皮细胞类型。对这些细胞中大多数细胞的角蛋白纤维进行免疫荧光染色，进一步证实它们为上皮细胞。
