壳聚糖基 miRNA 纳米颗粒促进大鼠间充质干细胞成骨。
Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles.
机构信息
State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China; College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China; Qingdao First Sanatorium, Jinan Military Region, No. 27 West Hong Kong Road, Qingdao 266071, Shandong Province, China.
College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China.
出版信息
Carbohydr Polym. 2016 Mar 15;138:49-58. doi: 10.1016/j.carbpol.2015.11.044. Epub 2015 Nov 19.
MicroRNAs (miRNAs) play important roles in the osteogenic differentiation of stem cells. However, the application of miRNA in bone regeneration has been limited by its poor stability, low cellular uptake, and undesired immune response. In this study, chitosan (CS)/tripolyphosphate (TPP)/Hyaluronic Acid (HA) nanoparticles (CTH NPs) were prepared to deliver antimiR-138 to bone marrow mesenchymal stem cells (MSCs). The particle size, polydispersity index, and zeta potential of CTH NPs were related to the weight ratio of CS:TPP:HA. At optimum N/P ratio (20:1), the highest encapsulation efficiency was obtained. Both blank CTH NPs and CTH/antmiR-138 NPs exhibited no cytotoxicity to MSCs. A high transfection efficiency (nearly 70%) and significant enhancement of the osteogenesis of MSCs were observed. Above results demonstrated that CTH NPs was a potential candidate as an efficient non-viral miRNA vector to regulate the osteogenic differentiation of MSCs.
微小 RNA（miRNA）在干细胞的成骨分化中发挥重要作用。然而，miRNA 在骨再生中的应用受到其稳定性差、细胞摄取率低和不理想的免疫反应的限制。在这项研究中，壳聚糖（CS）/三聚磷酸酯（TPP）/透明质酸（HA）纳米粒子（CTH NPs）被制备用于将抗 miR-138 递送至骨髓间充质干细胞（MSCs）。CTH NPs 的粒径、多分散指数和 zeta 电位与 CS:TPP:HA 的重量比有关。在最佳 N/P 比（20:1）下，获得了最高的包封效率。空白 CTH NPs 和 CTH/抗 miR-138 NPs 对 MSCs 均无细胞毒性。观察到高转染效率（接近 70%）和 MSC 成骨的显著增强。上述结果表明，CTH NPs 是一种有潜力的有效非病毒 miRNA 载体，可调节 MSCs 的成骨分化。
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