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大肠杆菌[甲酰胺嘧啶]DNA糖基化酶在无嘌呤/无嘧啶位点切割DNA链的机制。

Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase.

作者信息

Bailly V, Verly W G, O'Connor T, Laval J

机构信息

Laboratoire de Biochimie, Faculté des Sciences, Université de Liège, Belgium.

出版信息

Biochem J. 1989 Sep 1;262(2):581-9. doi: 10.1042/bj2620581.

DOI:10.1042/bj2620581
PMID:2679549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133308/
Abstract

Escherichia coli [formamidopyrimidine]DNA glycosylase catalyses the nicking of both the phosphodiester bonds 3' and 5' of apurinic or apyrimidinic sites in DNA so that the base-free deoxyribose is replaced by a gap limited by 3'-phosphate and 5'-phosphate ends. The two nickings are not the results of hydrolytic processes; the [formamidopyrimidine]DNA glycosylase rather catalyses a beta-elimination reaction that is immediately followed by a delta-elimination. The enzyme is without action on a 3'-terminal base-free deoxyribose or on a 3'-terminal base-free unsaturated sugar produced by a beta-elimination reaction nicking the DNA strand 3' to an apurinic or apyrimidinic site.

摘要

大肠杆菌[甲酰胺嘧啶]DNA糖基化酶催化DNA中无嘌呤或无嘧啶位点3'和5'处的磷酸二酯键断裂，使得无碱基的脱氧核糖被一个由3'-磷酸和5'-磷酸末端限定的缺口所取代。这两次断裂并非水解过程的结果；[甲酰胺嘧啶]DNA糖基化酶反而催化一个β-消除反应，该反应紧接着发生一个δ-消除反应。该酶对由β-消除反应在无嘌呤或无嘧啶位点3'端切断DNA链所产生的3'-末端无碱基脱氧核糖或3'-末端无碱基不饱和糖不起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/eadfae0c37a5/biochemj00200-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/3c9400a92ef9/biochemj00200-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/8db654d13636/biochemj00200-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/eadfae0c37a5/biochemj00200-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/3c9400a92ef9/biochemj00200-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/8db654d13636/biochemj00200-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c56c/1133308/eadfae0c37a5/biochemj00200-0202-a.jpg

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Eur J Biochem. 1983 Jan 1;129(3):509-17. doi: 10.1111/j.1432-1033.1983.tb07077.x.
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AP sites and AP endonucleases.脱嘌呤嘧啶位点与脱嘌呤嘧啶内切酶
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Structural biology of DNA abasic site protection by SRAP proteins.SRAP 蛋白对 DNA 无碱基位点的结构生物学保护。
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Excision of uracil from DNA by hSMUG1 includes strand incision and processing.hSMUG1 从 DNA 中切除尿嘧啶包括链切割和加工。
Nucleic Acids Res. 2019 Jan 25;47(2):779-793. doi: 10.1093/nar/gky1184.
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CiAPEX2 and CiP0, candidates of AP endonucleases in , have 3'-5' exonuclease activity and contribute to protection against oxidative stress.刺胞动物中脱嘌呤/脱嘧啶核酸内切酶的候选蛋白CiAPEX2和CiP0具有3'-5'核酸外切酶活性，并有助于抵御氧化应激。
Genes Environ. 2017 Dec 1;39:27. doi: 10.1186/s41021-017-0087-7. eCollection 2017.
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