Singsai Kanathip, Akaravichien Tarinee, Kukongviriyapan Veerapol, Sattayasai Jintana
Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Evid Based Complement Alternat Med. 2015;2015:970354. doi: 10.1155/2015/970354. Epub 2015 Dec 21.
This study investigated the effects of Streblus asper leaf extract (SA) on reactive oxygen species (ROS) in SK-N-SH cell culture and on motor functions and behaviors in MPTP-treated C57BL/6 mice. SK-N-SH cell viability after incubation with SA for 24 h was measured by MTT assay. Intracellular ROS levels of SK-N-SH cells were quantified after pretreatment with SA (0, 200, 600, and 1000 µg/mL) in the presence of H2O2 (300 µM). Male C57BL/6 mice were force-fed with water or 200 mg/kg/day SA for 32 days. Intraperitoneal injection of MPTP was used to induce Parkinson's disease-like symptoms. Catalepsy, beam balance ability, olfactory discrimination, social recognition, and spontaneous locomotor activity were assessed on days 19, 21, 23, 26, and 32, respectively. In cell culture, SA at 200, 600, and 1000 µg/mL significantly decreased ROS levels in H2O2-treated SK-N-SH cells. MPTP-treated C57BL/6 mice showed a significant change in all parameters tested when compared to the control group. Pretreatment and concurrent treatment with 200 mg/kg/day SA could antagonize the motor and cognitive function deficits induced by MPTP. The results show that SA possesses anti-Parkinson effects in MPTP-treated C57BL/6 mice and that reduction in ROS levels might be one of the mechanisms.
本研究调查了粗叶榕叶提取物（SA）对SK-N-SH细胞培养中活性氧（ROS）以及对经MPTP处理的C57BL/6小鼠运动功能和行为的影响。通过MTT法测定SA孵育24小时后SK-N-SH细胞的活力。在存在300µM H2O2的情况下，用SA（0、200、600和1000µg/mL）预处理后，对SK-N-SH细胞的细胞内ROS水平进行定量。雄性C57BL/6小鼠被强制喂水或200mg/kg/天的SA，持续32天。腹腔注射MPTP以诱导帕金森病样症状。分别在第19、21、23、26和32天评估僵住症、梁上平衡能力、嗅觉辨别、社会识别和自发运动活动。在细胞培养中，200、600和1000µg/mL的SA显著降低了H2O2处理的SK-N-SH细胞中的ROS水平。与对照组相比，经MPTP处理的C57BL/6小鼠在所有测试参数上均表现出显著变化。用200mg/kg/天的SA进行预处理和同时处理可拮抗MPTP诱导的运动和认知功能缺陷。结果表明，SA在经MPTP处理的C57BL/6小鼠中具有抗帕金森作用，并且ROS水平的降低可能是其中一种机制。
