Singsai Kanathip, Ladpala Natthanicha, Dangja Natthan, Boonchuen Thanyaret, Jaikhamfu Niracha, Fakthong Pirinyapat
Department of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, Thailand.
Unit of Excellence of Pharmacological Research and Vaccine Development in Animal Models, University of Phayao, Phayao 56000, Thailand.
Adv Pharmacol Pharm Sci. 2021 Apr 24;2021:6666726. doi: 10.1155/2021/6666726. eCollection 2021.
(SA) is well known as a folk medicinal plant in Asian countries. The effect of SA extract on preventing memory impairment in zebrafish induced by scopolamine was investigated. Male zebrafish, , were divided into 6 groups including the control, scopolamine 200 M (SCO), scopolamine plus rivastigmine 1.5 mg/kg (RV + SCO), and scopolamine plus SA extract at doses of 200, 400, and 800 mg/kg (SA200 + SCO, S400 + SCO, and SA800 + SCO), respectively. Spatial memory was evaluated by the colour-biased appetite conditioning T-maze test, while fear memory was measured by the inhibitory avoidance test. In the spatial memory test, results showed that the RV + SCO group had the best time spent ratio in the T-maze, followed by SA800 + SCO, SA400 + SCO, SA200 + SCO, control, and SCO group, respectively, but with no statistical significance. For the fear memory test, zebrafish that received SA at doses of 200, 400, and 800 mg/kg had significantly increased latency time as 21.75 ± 4.59, 23.75 ± 13.01, and 18.20 ± 18.84 min, respectively, when compared to the SCO group (9.80 ± 10.45 min). These results suggested that SA extract attenuated memory impairment in an inhibitory avoidance test related to fear memory. Our findings can be useful for further research to develop SA extract as a health product to ameliorate the symptoms of Alzheimer's disease.
在亚洲国家，刺蒺藜（SA）作为一种民间药用植物广为人知。本研究考察了刺蒺藜提取物对东莨菪碱诱导的斑马鱼记忆损伤的预防作用。雄性斑马鱼被分为6组，分别为对照组、200 μM东莨菪碱组（SCO）、1.5 mg/kg加兰他敏与东莨菪碱合用组（RV + SCO）以及200、400和800 mg/kg刺蒺藜提取物与东莨菪碱合用组（SA200 + SCO、SA400 + SCO和SA800 + SCO）。通过颜色偏好性食欲条件化T迷宫试验评估空间记忆，通过抑制性回避试验测量恐惧记忆。在空间记忆试验中，结果显示RV + SCO组在T迷宫中的停留时间比例最佳，其次分别为SA800 + SCO、SA400 + SCO、SA200 + SCO、对照组和SCO组，但无统计学意义。对于恐惧记忆试验，与SCO组（9.80 ± 10.45分钟）相比，接受200、400和800 mg/kg刺蒺藜的斑马鱼潜伏期时间显著增加，分别为21.75 ± 4.59、23.75 ± 13.01和18.20 ± 18.84分钟。这些结果表明，刺蒺藜提取物在与恐惧记忆相关的抑制性回避试验中减轻了记忆损伤。我们的研究结果可能有助于进一步研究开发刺蒺藜提取物作为一种保健品来改善阿尔茨海默病的症状。
