Barrow S E, Ward P S, Sleightholm M A, Ritter J M, Dollery C T
Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Biochim Biophys Acta. 1989 Oct 13;993(1):121-7. doi: 10.1016/0304-4165(89)90151-7.
Thromboxane (TX) B2, 2,3-dinor-TXB2, 11-dehydro-TXB2, 6-oxoprostaglandin (PG)F1 alpha and 2,3-dinor-6-oxo-PGF1 alpha were measured in 24 h urine samples obtained from 30 apparently healthy chronic cigarette smokers and 37 closely matched non-smoking control subjects. Samples were analysed using a newly developed assay based on immunoaffinity chromatography and capillary column gas chromatography/electron capture negative ion chemical ionisation mass spectrometry. There were significant and comparable increases in the excretion rates of both 2,3-dinor-TXB2 and 11-dehydro-TXB2 in the smoking compared with the non-smoking group (2P less than 0.001). Excretion rates of 2,3-dinor-TXB2 were 418 +/- 35 and 265 +/- 26 pg/mg creatinine in the two groups, respectively. 11-Dehydro-TXB2 excretion rates were 440 +/- 54 and 221 +/- 18 pg/mg creatinine, respectively (mean +/- S.E.). There were significant (2P less than 0.05) positive correlations between average reported cigarette consumption and excretion of both thromboxane metabolites. There were small but significant (2P less than 0.02) increases in the excretion rates of both 6-oxo-PGF1 alpha and 2,3-dinor-6-oxo-PGF1 alpha in the smoking compared with the non-smoking group. There was no significant difference in the rates of excretion of TXB2 in the two groups. The effects of acute cigarette smoke exposure (five cigarettes in 2 h) was also studied in four normally non-smoking healthy volunteers. There was no significant change in the excretion rate of any of the eicosanoids measured during control and smoking periods (at least 2 weeks apart), indicating that increased TXA2 biosynthesis in chronic smokers is unlikely to be a consequence of acute platelet activation.
在从30名表面健康的慢性吸烟者和37名匹配良好的非吸烟对照者获取的24小时尿液样本中，检测了血栓素（TX）B2、2,3 - 二去甲 - TXB2、11 - 脱氢 - TXB2、6 - 氧代前列腺素（PG）F1α和2,3 - 二去甲 - 6 - 氧代 - PGF1α。使用基于免疫亲和色谱和毛细管柱气相色谱/电子捕获负离子化学电离质谱的新开发检测方法对样本进行分析。与非吸烟组相比，吸烟组中2,3 - 二去甲 - TXB2和11 - 脱氢 - TXB2的排泄率均有显著且相当的增加（P<0.001）。两组中2,3 - 二去甲 - TXB2的排泄率分别为418±35和265±26 pg/mg肌酐。11 - 脱氢 - TXB2的排泄率分别为440±54和221±18 pg/mg肌酐（平均值±标准误）。平均报告的香烟消耗量与两种血栓素代谢物的排泄之间存在显著的正相关（P<0.05）。与非吸烟组相比，吸烟组中6 - 氧代 - PGF1α和2,3 - 二去甲 - 6 - 氧代 - PGF1α的排泄率均有小幅度但显著的增加（P<0.02）。两组中TXB2的排泄率无显著差异。还对4名正常不吸烟的健康志愿者进行了急性香烟烟雾暴露（2小时内吸5支烟）的影响研究。在对照期和吸烟期（至少间隔2周）期间，所测任何类二十烷酸的排泄率均无显著变化，这表明慢性吸烟者中TXA2生物合成增加不太可能是急性血小板活化的结果。
