Liu D Q, Ren D X, Liu R J, Qiu C P, Gao D Q
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1989;7(2):112-4.
The use of liquid RPMI 1640 medium (added NaHCO3 and serum) in sealed ampoule for in vitro assessment of sensitivity of Plasmodium falciparum to chloroquine and meflopuine in the field was very successful. On chloroquine plates with WHO supplied medium, 9 of 13 isolates were interpretable (successful rate 69.2%), the maturation rate from ring-forms to schizonts was 53.3%, the average number of nuclei per schizont 5.1. With lyophilized medium the successful rate was 100%, the maturation rate 65.9%, the average number of nuclei per schizont 7.4, while with medium in sealed ampoule, the corresponding figures were 92.3%, 65.1% and 7.3 respectively. On mefloquine plates with WHO supplied medium, 7 of 11 isolates were interpretable (63.6%). In control wells, 52.3% of schizonts matured, the number of nuclei per schizont was 5.1. With lyophilized medium the successful rate was 100%, the maturation rate 61.9%, the number of nuclei was 8.1, while those with liquid medium in sealed ampoule were 100%, 59.8% and 7.5 respectively. The results showed that the liquid medium in sealed ampoules stored within 56 days at 4 degrees C could still support the growth of Plasmodium falciparum, its supporting effect being better than that of WHO standard medium, but similar to lyophilized medium. The liquid medium in sealed ampoule had the advantages of easy carrying time-saving and more applicability under field conditions.
在现场使用密封安瓿中的液体RPMI 1640培养基（添加了NaHCO₃和血清）对恶性疟原虫进行氯喹和甲氟喹体外敏感性评估非常成功。在使用世界卫生组织提供的培养基的氯喹平板上，13个分离株中有9个可进行分析（成功率69.2%），从环状体到裂殖体的成熟率为53.3%，每个裂殖体的平均核数为5.1。使用冻干培养基时，成功率为100%，成熟率为65.9%，每个裂殖体的平均核数为7.4，而使用密封安瓿中的培养基时，相应数字分别为92.3%、65.1%和7.3。在使用世界卫生组织提供的培养基的甲氟喹平板上，11个分离株中有7个可进行分析（63.6%）。在对照孔中，52.3%的裂殖体成熟，每个裂殖体的核数为5.1。使用冻干培养基时，成功率为100%，成熟率为61.9%，核数为8.1，而使用密封安瓿中的液体培养基时分别为100%、59.8%和7.5。结果表明，密封安瓿中的液体培养基在4℃下储存56天内仍能支持恶性疟原虫的生长，其支持效果优于世界卫生组织标准培养基，但与冻干培养基相似。密封安瓿中的液体培养基具有携带方便、节省时间以及在现场条件下适用性更强的优点。
