Yang H, Liu D, Huang K, Yang Y, Yang P, Liao M, Zhang C
Yunnan Institute of Malaria Control, Simao 665000.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(1):43-5.
To determine the sensitivity of P. falciparum to chloroquine, amodiaquine, piperaquine, mefloquine and quinine in Yunnan Province of China in 1992-1995.
Rieckmann's in vitro microtechnigue was used. The sensitivity of P. falciparum was tested to the above-mentioned antimalarials.
The resistance rates of isolates of P. falciparum from the south, southeast and the west part of Yunnan to chloroquine and amodiaquine were 96.7% (29/30), 78.9% (30/38), 95.7% (22/23) and 100% (30/30), 85.3% (29/34), 8/9, respectively, with their corresponding ID50 of 125 nmol/L, 136 nmol/L and 176 nmol/L, and 52 nmol/L, 64 nmol/L and 72 nmol/L, respectively. All the isolates were sensitive to quinine and their ID50 were 480 nmol/L, 352 nmol/L, 608 nmol/L, respectively. The resistance rates of P. falciparum from the south part and southeast part of Yunnan to piperaquine were 96.4% (27/29), 72.9 (27/37), respectively, their ID50 were 320 nmol/L and 228 nmol/L; all the cases were sensitive to mefloqine, their ID50 were 68 nmol/L and 88 nmol/L.
P. falciparum generally produces resistance to chloroquine, amodiaquine and piperaquine in Yunnan Province; the degree of resistance to chloroquine of P. falciparum from the west part of Yunnan were higher than the P. falciparum from the southeast part of Yunnan; all the isolates were sensitive to mefloquine and quinine in this region.
确定1992 - 1995年中国云南省恶性疟原虫对氯喹、氨酚喹、哌喹、甲氟喹和奎宁的敏感性。
采用里克曼体外微量技术，检测恶性疟原虫对上述抗疟药的敏感性。
云南省南部、东南部和西部的恶性疟原虫分离株对氯喹和氨酚喹的耐药率分别为96.7%（29/30）、78.9%（30/38）、95.7%（22/23）和100%（30/30）、85.3%（29/34）、8/9，其相应的半数抑制浓度（ID50）分别为125纳摩尔/升、136纳摩尔/升和176纳摩尔/升，以及52纳摩尔/升、64纳摩尔/升和72纳摩尔/升。所有分离株对奎宁敏感，其ID50分别为480纳摩尔/升、352纳摩尔/升、608纳摩尔/升。云南省南部和东南部的恶性疟原虫对哌喹的耐药率分别为96.4%（27/29）、72.9%（27/37），其ID50分别为320纳摩尔/升和228纳摩尔/升；所有病例对甲氟喹敏感，其ID50分别为68纳摩尔/升和88纳摩尔/升。
云南省恶性疟原虫对氯喹、氨酚喹和哌喹普遍产生耐药性；云南省西部恶性疟原虫对氯喹的耐药程度高于云南省东南部的恶性疟原虫；该地区所有分离株对甲氟喹和奎宁敏感。
