Zhang Ling, Gan Xueqi, Zhu Zhuoli, Yang Yang, He Yuting, Yu Haiyang
Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
Biochem Biophys Res Commun. 2016 Feb 12;470(3):510-515. doi: 10.1016/j.bbrc.2016.01.120. Epub 2016 Jan 21.
Although many previous studies have shown that refractory period-dependent memory effect of vibration stress is anabolic for skeletal homeostasis, little is known about the rapid response of osteoblasts simply derived from vibration itself. In view of the potential role of reactive oxygen species (ROS) in mediating differentiated activity of osteoblasts, whether and how ROS regulates the rapid effect of vibration deserve to be demonstrated. Our findings indicated that MC3T3-E1 cells underwent decreased gene expression of Runx2, Col-I and ALP and impaired ALP activity accompanied by increased mitochondrial fission immediately after vibration loading. Moreover, we also revealed the involvement of ERK-Drp1 signal transduction in ROS regulatory mechanisms responsible for the rapid effect of vibration stress.
尽管此前许多研究表明，振动应激的不应期依赖性记忆效应对于骨骼稳态具有合成代谢作用，但对于单纯由振动本身引起的成骨细胞快速反应却知之甚少。鉴于活性氧（ROS）在介导成骨细胞分化活性中的潜在作用，ROS是否以及如何调节振动的快速效应值得研究。我们的研究结果表明，振动加载后，MC3T3-E1细胞立即出现Runx2、Col-I和ALP基因表达降低，ALP活性受损，同时线粒体分裂增加。此外，我们还揭示了ERK-Drp1信号转导参与了负责振动应激快速效应的ROS调节机制。
