Zhuoli Zhu, Ling Zhang, Ruiyang Ma, Xueqi Gan
State Key Laboratory of Oral Diseases, Dept. of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Feb 1;35(1):68-72. doi: 10.7518/hxkq.2017.01.010.
This study investigated the rapid response of osteoblasts, which were derived from low-magnitude high-frequency vibration (LMHFV). Refractory period-derived memory response was also observed.
MC3T3-E1 cells were incubated and received LMHFV stimulation (0.49 g, 40 Hz) for 30 min. After application of LMHFV, mRNA levels of earlier osteogenic differentiation markers Runt-related transcription factor 2 (Runx2), collagen typeⅠ(Col-Ⅰ), and alkaline phosphatase (ALP) were immediately detected by real-time fluorescence quantitative polymerase chain reaction in the absence or presence of antioxidant. Simultaneously, concentrations of mitochondrial reactive oxygen species (ROS) and average mitochondrial length were also measured.
Osteoblasts in the vibration group showed decreased gene expressions of Runx2, Col-Ⅰ, and ALP (P<0.01) and increased levels of mitochondrial ROS (P<0.01) and shortened mitochondria (P<0.01), whereas antioxidant treatment resulted in recovery from changes in the above indicators (P<0.01).
CONCLUSIONS: LMHFV can downregulate mRNA levels of early osteogenic differentiation markers, promote ROS generation, and mitochondrial fission. .
本研究调查了源自低强度高频振动（LMHFV）的成骨细胞的快速反应。还观察到了不应期衍生的记忆反应。
培养MC3T3-E1细胞并给予LMHFV刺激（0.49 g，40 Hz）30分钟。施加LMHFV后，在存在或不存在抗氧化剂的情况下，通过实时荧光定量聚合酶链反应立即检测早期成骨分化标志物Runx2相关转录因子2（Runx2）、Ⅰ型胶原蛋白（Col-Ⅰ）和碱性磷酸酶（ALP）的mRNA水平。同时，还测量了线粒体活性氧（ROS）的浓度和线粒体平均长度。
振动组中的成骨细胞显示Runx2、Col-Ⅰ和ALP的基因表达降低（P<0.01），线粒体ROS水平升高（P<0.01），线粒体缩短（P<0.01），而抗氧化剂处理导致上述指标的变化恢复（P<0.01）。
LMHFV可下调早期成骨分化标志物的mRNA水平，促进ROS生成和线粒体分裂。
