College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China.
Hunan East Dongting Lake National Nature Reserve Administration, Yueyang 414000, China.
J Environ Sci (China). 2016 May;43:40-47. doi: 10.1016/j.jes.2015.08.020. Epub 2016 Jan 6.
Aquatic macrophytes are considered to be promising in controlling harmful cyanobacterial blooms. In this research, an aqueous extract of Sagittaria trifolia tubers was prepared to study its inhibitory effect on Microcystis aeruginosa in the laboratory. Several physiological indices of M. aeruginosa, in response to the environmental stress, were analyzed. Results showed that S. trifolia tuber aqueous extract significantly inhibited the growth of M. aeruginosa in a concentration-dependent way. The highest inhibition rate reached 90% after 6 day treatment. The Chlorophyll-a concentration of M. aeruginosa cells decreased from 343.1 to 314.2μg/L in the treatment group. The activities of superoxide dismutase and peroxidase and the content of reduced glutathione in M. aeruginosa cells initially increased as a response to the oxidative stress posed by S. trifolia tuber aqueous extract, but then decreased as time prolonged. The lipid peroxidation damage of the cyanobacterial cell membranes was reflected by the malondialdehyde level, which was notably higher in the treatment group compared with the controls. It was concluded that the oxidative damage of M. aeruginosa induced by S. trifolia tuber aqueous extract might be one of the mechanisms for the inhibitory effects.
水生植物被认为在控制有害蓝藻水华方面具有很大的潜力。在这项研究中，制备了慈姑块茎的水提物，以研究其对铜绿微囊藻在实验室中的抑制作用。分析了铜绿微囊藻对环境胁迫的几种生理指标。结果表明，慈姑块茎水提物对铜绿微囊藻的生长具有浓度依赖性的显著抑制作用。处理 6 天后，最高抑制率达到 90%。实验组铜绿微囊藻细胞中的叶绿素 a 浓度从 343.1μg/L 降至 314.2μg/L。铜绿微囊藻细胞中超氧化物歧化酶和过氧化物酶的活性以及还原型谷胱甘肽的含量最初会因慈姑块茎水提物引起的氧化应激而增加，但随着时间的延长而减少。丙二醛水平反映了蓝藻细胞膜的脂质过氧化损伤，实验组明显高于对照组。结论：慈姑块茎水提物诱导铜绿微囊藻的氧化损伤可能是其抑制作用的机制之一。
