Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.
J Nat Prod. 2016 Feb 26;79(2):387-94. doi: 10.1021/acs.jnatprod.5b01053. Epub 2016 Jan 26.
Eleven new abietane-type diterpenes, holophyllins D-N (1-11), and 17 known analogues (12-28), were isolated from a MeOH extract of the trunk of Abies holophylla. The chemical structures of 1-11 were determined through spectroscopic data analysis, including NMR ((1)H and (13)C NMR, DEPT, (1)H-(1)H COSY, HMQC, HMBC, and NOESY) and HRFABMS methods. All isolated compounds (1-28) were evaluated for their cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-116), for their potential neuroprotective effects through induction of nerve growth factor in C6 glioma cells, and for their effects on nitric oxide levels in lipopolysaccharide-stimulated murine microglia BV2 cells.
从国产冷杉树干的甲醇提取物中分离得到了 11 个新的扁柏烷型二萜类化合物，即霍朴林 D-N（1-11），以及 17 个已知类似物（12-28）。通过包括 NMR（（1）H 和（13）C NMR、DEPT、（1）H-（1）H COSY、HMQC、HMBC 和 NOESY）和 HRFABMS 在内的光谱数据分析确定了 1-11 的化学结构。所有分离得到的化合物（1-28）都进行了人源肿瘤细胞系（A549、SK-OV-3、SK-MEL-2 和 HCT-116）的细胞毒性评价、通过诱导 C6 神经胶质瘤细胞产生神经生长因子的潜在神经保护作用评价，以及对脂多糖刺激的小鼠小胶质细胞 BV2 细胞中一氧化氮水平的影响评价。
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