Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 440-746, Republic of Korea.
J Nat Prod. 2013 Nov 22;76(11):2131-5. doi: 10.1021/np4005322. Epub 2013 Nov 13.
Six new lignans (1-6) were isolated from the trunk of Abies holophylla MAXIM, together with 11 known lignans (7-17). The structures of 1-7 were elucidated by spectroscopic methods, acid hydrolysis, and use of the modified Mosher's method. The effects of the isolates on nerve growth factor induction in a C6 rat glioma cell line were evaluated. Compounds 6, 7, and 13 showed significant induction of nerve growth factor secretion at concentrations of 10 μM. Compounds 1, 5, 6, and 16 showed moderate inhibitory effects on nitric oxide production in lipopolysaccharide-activated BV-2 cells (IC50 28.5-36.4 μM).
从巴山冷杉树干中分离得到 6 个新的木脂素（1-6），并结合 11 个已知的木脂素（7-17）。通过光谱方法、酸水解和改进的 Mosher 法，确定了 1-7 的结构。对分离物在 C6 大鼠神经胶质瘤细胞系中诱导神经生长因子的作用进行了评价。在 10 μM 的浓度下，化合物 6、7 和 13 显示出对神经生长因子分泌的显著诱导作用。化合物 1、5、6 和 16 对脂多糖激活的 BV-2 细胞中一氧化氮产生具有中等抑制作用（IC50 28.5-36.4 μM）。
