Forestry College, Henan University of Science & Technology, Luoyang, 471000, China.
Guangyuan Tobacco Corporation of Sichuan Province, Guangyuan, 628000, China.
Mol Biol Rep. 2021 Apr;48(4):3127-3143. doi: 10.1007/s11033-021-06327-8. Epub 2021 Apr 19.
Chemosensory receptors in the dendritic membrane of olfactory cells are critical for the molecular recognition and discrimination of odorants. Tropidothorax elegans is a major pest of agricultural, ornamental, and medicinal plants. However, very little is known about olfactory genes in T. elegans. The purpose of this study was to obtain chemosensory receptor genes by sequencing the antennal transcriptome of T. elegans using Illumina sequencing technology. We identified 153 candidate chemosensory receptors, including 121 olfactory receptors (including one olfactory receptor co-receptor), 10 ionotropic receptors (including one IR8a and one IR25a), and 22 gustatory receptors (GRs). TeleOR76, 104 and 112 displayed more highly expression level than TeleOrco. Other TeleGR genes were expressed at very low levels except TeleGR1 and 20. TeleIR76b was the most highly expressed among TeleIR genes. Our results provide valuable biological information for studies of the olfactory communication system of T. elegans.
化感感受器在嗅觉细胞的树突膜中对于气味物质的分子识别和区分至关重要。秀丽隐杆线虫是农业、观赏和药用植物的主要害虫。然而，关于秀丽隐杆线虫的嗅觉基因知之甚少。本研究旨在通过 Illumina 测序技术对秀丽隐杆线虫的触角转录组进行测序，获得化感受体基因。我们鉴定了 153 个候选化感受体，包括 121 个嗅觉受体（包括一个嗅觉受体共受体）、10 个离子型受体（包括一个 IR8a 和一个 IR25a）和 22 个味觉受体（GRs）。TeleOR76、104 和 112 的表达水平高于 TeleOrco。除了 TeleGR1 和 20 外，其他 TeleGR 基因的表达水平非常低。TeleIR76b 是 TeleIR 基因中表达水平最高的。我们的研究结果为秀丽隐杆线虫嗅觉通讯系统的研究提供了有价值的生物学信息。
