Department of Chemical Engineering, Process and Environmental Technology Lab, KU Leuven, Technology Campus De Nayer, J. De Nayerlaan 5, B-2860, Sint-Katelijne-Waver, Belgium.
Department of Microbial and Molecular Systems (M2S), Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, B-2860, Sint-Katelijne-Waver, Belgium.
Appl Microbiol Biotechnol. 2016 Jun;100(12):5339-52. doi: 10.1007/s00253-016-7321-2. Epub 2016 Jan 27.
Comparative analyses of bacterial and archaeal community structures and dynamics in three biogas digesters during start-up and subsequent operation using microwaved, ultrasonicated or untreated waste activated sludge were performed based on 454 pyrosequencing datasets of part of 16S ribosomal RNA sequences and quantitative PCR. The pre-treatment increased the solubility, and thus the availability of the substrate for microbial degradation and significantly affected the succession of the anaerobic community structure over the course of the digestion. Bacteroidetes, Proteobacteria and Firmicutes were the dominant phyla in all digesters throughout operation. Proteobacteria decreased in relative abundance from 23-26 % to 11-13 % in association with enhanced substrate availability. Negative correlations between relative abundance of Alpha-, Beta- and Gammaproteobacteria and the substrate availability and/or biogas production were disclosed in statistical analyses. Clostridiales was the dominant order in Firmicutes, and Clostridiales, Clostridia and Firmicutes relative abundance and richness were shown to positively correlate with substrate availability and biogas generation. Methanogenic communities had a fairly restricted structure, highly dominated by Methanosaeta and Methanobrevibacter phylotypes. A gradual decline in Methanobrevibacter and increased representation of Methanosaeta concilii over time were particularly apparent in the digester receiving untreated waste activated sludge, whereas more diversified archaeal communities were maintained in the pre-treatment digesters. The quantitative PCR analyses revealed a methanogenic community distribution that coincided with the 454 pyrosequencing data.
基于部分 16S 核糖体 RNA 序列和定量 PCR 的 454 焦磷酸测序数据集，对使用微波、超声或未经处理的废活性污泥预处理启动和后续运行的三个沼气消化器中的细菌和古菌群落结构和动态进行了比较分析。预处理增加了底物的溶解性，从而提高了微生物降解的底物可用性，并在消化过程中显著影响了厌氧群落结构的演替。在整个运行过程中，拟杆菌门、变形菌门和厚壁菌门是所有消化器中的主要门。随着底物可用性的提高，相对丰度从 23-26%减少到 11-13%。统计分析揭示了 Alpha-、Beta-和 Gamma-变形菌的相对丰度与底物可用性和/或沼气产量之间存在负相关关系。在厚壁菌门中，梭菌目是主要目，梭菌目、梭菌纲和厚壁菌纲的相对丰度和丰富度与底物可用性和沼气生成呈正相关。产甲烷菌群落结构相当受限，高度由 Methanosaeta 和 Methanobrevibacter 型主导。在接收未经处理的废活性污泥的消化器中，Methanobrevibacter 逐渐减少，Methanosaeta concilii 的代表性增加，这一点尤其明显，而预处理消化器中保持了更多样化的古菌群落。定量 PCR 分析揭示了与 454 焦磷酸测序数据相吻合的产甲烷菌群落分布。
