Institute for regenerative medicine and biomimetic materials, School of materials science and engineering, Tsinghua University, Beijing 100084, China, Department of anatomy, histology and embryology, School of basic medical sciences, Capital Medical University, Beijing 100069, China and Department of material science and chemical engineering, Hainan University, Haikou 570228, China.
Regen Biomater. 2014 Nov;1(1):37-47. doi: 10.1093/rb/rbu012. Epub 2014 Oct 20.
To declare the mechanisms of neural stem cells (NSCs) in response to material surface chemistry, NSCs were exposed to the self-assemble monolayers of alkanethiolates on gold surfaces terminated with amine (NH2), hydroxyl (OH) and methyl (CH3) for analysis. The morphological responses of NSCs were recorded; the gene expression profilings were detected by genechips; the gene expressions data of NSCs responded to different chemical groups were declared through the gene ontology term and pathway analyses. It showed that cells behaved dissimilar on the three chemical groups, the adhesion, proliferation and migration were easier on the NH2 and OH groups; the gene expressions of NSCs were induced differently, either, involved in several functional processes and signaling pathways. CH3 group induced genes enriched much in chemistry reactions and death processes, whereas many genes of cellular nucleotide metabolism were down-regulated. NH2 group induced NSCs to express many genes of receptors on membrane, and participated in cellular signal transduction of cell adhesion and interactions, or associated with axon growth. OH group was similar to NH2 group to induce the membrane response, but it also down regulated metabolism of cells. Therefore, it declared the chemical groups affected NSCs through inner way and the NH2, OH and CH3 groups triggered the cellular gene expression in different signaling pathways.
为了阐明神经干细胞（NSCs）对材料表面化学性质的反应机制，我们将 NSCs 暴露于金表面上带有胺基（NH2）、羟基（OH）和甲基（CH3）的自组装单分子层烷硫醇中进行分析。记录 NSCs 的形态反应；通过基因芯片检测基因表达谱；通过基因本体论术语和途径分析，阐明 NSCs 对不同化学基团的基因表达数据。结果表明，细胞在这三种化学基团上的表现不同，NH2 和 OH 基团上细胞的黏附、增殖和迁移更容易；NSCs 的基因表达也受到不同的诱导，涉及到几个功能过程和信号通路。CH3 基团诱导的基因在化学反应和死亡过程中富集较多，而细胞核苷酸代谢的许多基因则下调。NH2 基团诱导 NSCs 表达许多膜受体基因，并参与细胞黏附和相互作用的细胞信号转导，或与轴突生长有关。OH 基团与 NH2 基团相似，可诱导膜反应，但也可下调细胞代谢。因此，它声明化学基团通过内在途径影响 NSCs，而 NH2、OH 和 CH3 基团则在不同的信号通路中触发细胞基因表达。
