Lopez-Minguez J, Colodro-Conde L, Bandín C, Ordoñana J R, Garaulet M, Madrid J A
a Department of Physiology, Faculty of Biology , University of Murcia , Murcia , Spain.
b IMIB-Arrixaca , Murcia , Spain.
Chronobiol Int. 2016;33(2):234-44. doi: 10.3109/07420528.2015.1130051. Epub 2016 Jan 28.
At present, the measurement of circadian system status under free-living conditions by the use of sensors is a relatively new technique. The data obtained using these methods are influenced by strong environmental masking factors and artifacts that can affect its recording. Therefore, the use of integrative variables such as TAP, a measure that includes temperature, activity and position that reduces these drawbacks and the number of parameters obtained is necessary. However, the relative genetic contribution to this circadian marker is unknown. The aim of our study was to ascertain the relative importance of genetic influences in TAP, and for each of its components using classical twin models. The study was performed in 53 pairs of female twins [28 monozygotic (MZ) and 25 dizygotic (DZ)] with mean age 52 ± 6 years. Circadian patterns were studied by analyzing temperature, body position and activity for 1 week every 1 min with "Circadianware®.". Genetic influences affecting the variability of each of the measurements were estimated by comparing the observed data in twin pairs. MZ twins showed higher intrapair correlations than DZ twins for most of the parameters. Genetic factors (broad sense heritability) were responsible for about 40-72% of TAP variance in parameters such as mesor, acrophase, amplitude, Rayleigh test, percentage of rhythmicity and circadian function index. We found more homogeneous heritability estimates of the circadian system when using an integrative technique such as TAP than with individual variables alone, suggesting that this measurement can be more reliable and less subject to environmental artifacts.
目前，利用传感器在自由生活条件下测量昼夜节律系统状态是一项相对较新的技术。使用这些方法获得的数据会受到强烈的环境掩盖因素和可能影响其记录的伪影的影响。因此，使用诸如TAP这样的综合变量是必要的，TAP是一种包含温度、活动和位置的测量方法，它减少了这些缺点以及所获得的参数数量。然而，这种昼夜节律标志物的相对遗传贡献尚不清楚。我们研究的目的是使用经典双生子模型确定遗传影响在TAP及其每个组成部分中的相对重要性。该研究在53对平均年龄为52±6岁的女性双胞胎[28对同卵双胞胎（MZ）和25对异卵双胞胎（DZ）]中进行。通过使用“Circadianware®”每1分钟分析温度、身体位置和活动1周来研究昼夜节律模式。通过比较双胞胎对中的观察数据来估计影响每个测量值变异性的遗传影响。对于大多数参数，MZ双胞胎显示出比DZ双胞胎更高的对内相关性。在诸如中值、峰相位、振幅、瑞利检验、节律性百分比和昼夜节律功能指数等参数中，遗传因素（广义遗传力）约占TAP方差的40 - 72%。我们发现，与单独使用个体变量相比，使用诸如TAP这样的综合技术时，昼夜节律系统的遗传力估计更具同质性，这表明这种测量可能更可靠，受环境伪影的影响更小。
