Mansour Joseph M, Lee Zhenghong, Welter Jean F
Departments of Mechanical and Aerospace Engineering, Case Western Reserve University, 2123 Martin Luther King Jr. Drive, Glennan Building Room 616A, Cleveland, OH, 44106, USA.
Radiology and Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA.
Ann Biomed Eng. 2016 Mar;44(3):733-49. doi: 10.1007/s10439-015-1535-9. Epub 2016 Jan 27.
In this review, methods for evaluating the properties of tissue engineered (TE) cartilage are described. Many of these have been developed for evaluating properties of native and osteoarthritic articular cartilage. However, with the increasing interest in engineering cartilage, specialized methods are needed for nondestructive evaluation of tissue while it is developing and after it is implanted. Such methods are needed, in part, due to the large inter- and intra-donor variability in the performance of the cellular component of the tissue, which remains a barrier to delivering reliable TE cartilage for implantation. Using conventional destructive tests, such variability makes it near-impossible to predict the timing and outcome of the tissue engineering process at the level of a specific piece of engineered tissue and also makes it difficult to assess the impact of changing tissue engineering regimens. While it is clear that the true test of engineered cartilage is its performance after it is implanted, correlation of pre and post implantation properties determined non-destructively in vitro and/or in vivo with performance should lead to predictive methods to improve quality-control and to minimize the chances of implanting inferior tissue.
在本综述中，描述了评估组织工程化（TE）软骨特性的方法。其中许多方法是为评估天然和骨关节炎关节软骨的特性而开发的。然而，随着对软骨工程的兴趣日益增加，需要专门的方法来对正在发育的组织及其植入后进行无损评估。之所以需要这些方法，部分原因是组织细胞成分的性能在供体间和供体内存在很大差异，这仍然是为植入提供可靠的TE软骨的一个障碍。使用传统的破坏性测试，这种变异性使得几乎不可能在特定工程组织水平上预测组织工程过程的时间和结果，也难以评估改变组织工程方案的影响。虽然很明显，对工程化软骨的真正测试是其植入后的性能，但在体外和/或体内无损测定的植入前后特性与性能之间的相关性应能产生预测方法，以改进质量控制并尽量减少植入劣质组织的可能性。
