Trachtenberg Jordan E, Vo Tiffany N, Mikos Antonios G
Department of Bioengineering, Rice University, MS 142, P.O. Box 1892, Houston, TX, 77251-1892, USA.
Ann Biomed Eng. 2015 Mar;43(3):681-96. doi: 10.1007/s10439-014-1151-0. Epub 2014 Oct 16.
Pre-clinical animal models play a crucial role in the translation of biomedical technologies from the bench top to the bedside. However, there is a need for improved techniques to evaluate implanted biomaterials within the host, including consideration of the care and ethics associated with animal studies, as well as the evaluation of host tissue repair in a clinically relevant manner. This review discusses non-invasive, quantitative, and real-time techniques for evaluating host-materials interactions, quality and rate of neotissue formation, and functional outcomes of implanted biomaterials for bone and cartilage tissue engineering. Specifically, a comparison will be presented for pre-clinical animal models, histological scoring systems, and non-invasive imaging modalities. Additionally, novel technologies to track delivered cells and growth factors will be discussed, including methods to directly correlate their release with tissue growth.
临床前动物模型在将生物医学技术从实验室台面转化到临床应用中起着至关重要的作用。然而，需要改进技术来评估宿主体内植入的生物材料，这包括考虑与动物研究相关的护理和伦理问题，以及以临床相关的方式评估宿主组织修复情况。本综述讨论了用于评估宿主-材料相互作用、新组织形成的质量和速率以及骨和软骨组织工程中植入生物材料的功能结果的非侵入性、定量和实时技术。具体而言，将对临床前动物模型、组织学评分系统和非侵入性成像模式进行比较。此外，还将讨论追踪递送的细胞和生长因子的新技术，包括将它们的释放与组织生长直接关联的方法。
