Arca-Ramos A, Kumar V V, Eibes G, Moreira M T, Cabana H
Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, 603203, India.
Environ Sci Pollut Res Int. 2016 May;23(9):8929-39. doi: 10.1007/s11356-016-6139-x. Epub 2016 Jan 28.
In the present work, the use of magnetic mesoporous silica microbeads (MMSMB) as supports was proposed to produce magnetically-separable cross-linked enzyme aggregates (MCLEAs). The effects of cross linking time, addition of bovine serum albumin as protein feeder, pH, glutaraldehyde concentration, and laccase:MMSMB mass ratio on the immobilization yield and enzyme load were investigated. The best conditions allowed the rapid preparation of MCLEAs with high enzyme load, i.e., 1.53 U laccase/mg MCLEAs. The stability of MCLEAs was improved with regard to low pH, presence of chemical denaturants, and real wastewater matrix, compared to free laccase. In addition, the novel biocatalyst exhibited good operational stability, maintaining up to 70 % of its initial activity after 10 successive batch reactions. Finally, MCLEAs demonstrated its catalytic potential to transform acetaminophen and various non-phenolic pharmaceutical active compounds as mefenamic acid, fenofibrate, and indomethacin from biologically treated wastewater effluent, with similar or even higher efficiency than free laccase.
在本研究中，提出使用磁性介孔二氧化硅微珠（MMSMB）作为载体来制备磁分离交联酶聚集体（MCLEA）。研究了交联时间、添加牛血清白蛋白作为蛋白质供体、pH值、戊二醛浓度以及漆酶与MMSMB的质量比对固定化产率和酶负载量的影响。最佳条件下能够快速制备出具有高酶负载量的MCLEA，即1.53 U漆酶/mg MCLEA。与游离漆酶相比，MCLEA在低pH值、存在化学变性剂以及实际废水基质的情况下稳定性得到了提高。此外，这种新型生物催化剂表现出良好的操作稳定性，在连续10次分批反应后仍保持其初始活性的70%。最后，MCLEA展示了其催化潜力，能够将对乙酰氨基酚以及各种非酚类药物活性化合物（如甲芬那酸、非诺贝特和吲哚美辛）从生物处理后的废水流出物中转化出来，其效率与游离漆酶相似甚至更高。
