Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, 101-E-17003 Girona, Spain.
Departament d'Enginyeria Química, Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
Chemosphere. 2015 Oct;136:9-19. doi: 10.1016/j.chemosphere.2015.03.051. Epub 2015 Apr 22.
This work presents a study about the elimination of anticancer drugs, a group of pollutants considered recalcitrant during conventional activated sludge wastewater treatment, using a biological treatment based on the fungus Trametes versicolor. A 10-L fluidized bed bioreactor inoculated with this fungus was set up in order to evaluate the removal of 10 selected anticancer drugs in real hospital wastewater. Almost all the tested anticancer drugs were completely removed from the wastewater at the end of the batch experiment (8 days) with the exception of Ifosfamide and Tamoxifen. These two recalcitrant compounds, together with Cyclophosphamide, were selected for further studies to test their degradability by T. versicolor under optimal growth conditions. Cyclophosphamide and Ifosfamide were inalterable during batch experiments both at high and low concentration, whereas Tamoxifen exhibited a decrease in its concentration along the treatment. Two positional isomers of a hydroxylated form of Tamoxifen were identified during this experiment using a high resolution mass spectrometry based on ultra-high performance chromatography coupled to an Orbitrap detector (LTQ-Velos Orbitrap). Finally the identified transformation products of Tamoxifen were monitored in the bioreactor run with real hospital wastewater.
本研究利用木蹄层孔菌（Trametes versicolor）开展了一项生物处理技术研究，以去除传统活性污泥法处理废水时难以去除的一类污染物——抗癌药物。为此，我们构建了一个 10L 的流化床生物反应器，并将该真菌接种到其中，以评估其对实际医院废水中 10 种选定的抗癌药物的去除效果。在批处理实验（8 天）结束时，除异环磷酰胺和他莫昔芬外，几乎所有测试的抗癌药物都被完全从废水中去除。这两种难降解的化合物，以及环磷酰胺，被选择用于进一步研究，以测试它们在真菌最佳生长条件下的可降解性。在高浓度和低浓度下，环磷酰胺和异环磷酰胺在批处理实验中都没有变化，而他莫昔芬的浓度则随着处理的进行而降低。在这个实验中，使用基于超高效色谱法与轨道阱高分辨质谱仪联用的方法（LTQ-Velos Orbitrap），鉴定出了他莫昔芬的一种羟基化形式的两个位置异构体。最后，在使用实际医院废水运行的生物反应器中监测到了他莫昔芬的转化产物。
