School of Environmental Science and Engineering, POSTECH, Pohang 790-784, Republic of Korea.
Department of Environmental Science, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India.
Bioresour Technol. 2016 Sep;216:203-10. doi: 10.1016/j.biortech.2016.05.077. Epub 2016 May 21.
Immobilization of laccase has been highlighted to enhance their stability and reusability in bioremediation. In this study, we provide a novel immobilization technique that is very suitable to real wastewater treatment. A perfect core-shell system composing copper alginate for the immobilization of laccase (Lac-beads) was produced. Additionally, nFe2O3 was incorporated for the bead recycling through magnetic force. The beads were proven to immobilize 85.5% of total laccase treated and also to be structurally stable in water, acetate buffer, and real wastewater. To test the Lac-beads reactivity, triclosan (TCS) and Remazol Brilliant Blue R (RBBR) were employed. The Lac-beads showed a high percentage of TCS removal (89.6%) after 8h and RBBR decolonization at a range from 54.2% to 75.8% after 4h. Remarkably, the pollutants removal efficacy of the Lac-beads was significantly maintained in real wastewater with the bead recyclability, whereas that of the corresponding free laccase was severely deteriorated.
固定化漆酶已被强调用于提高其在生物修复中的稳定性和可重复使用性。在这项研究中，我们提供了一种非常适合实际废水处理的新型固定化技术。我们制备了一种由海藻酸钠组成的完美的核壳系统，用于固定化漆酶（Lac-beads）。此外，还加入了 nFe2O3 以通过磁力进行珠粒回收。实验证明，这些珠粒可以固定 85.5%的总漆酶，并且在水中、乙酸盐缓冲液和实际废水中结构稳定。为了测试 Lac-beads 的反应性，我们使用了三氯生（TCS）和雷马唑尔亮蓝 R（RBBR）。Lac-beads 在 8 小时后显示出 TCS 去除率（89.6%）非常高，在 4 小时后 RBBR 脱色率为 54.2%至 75.8%。值得注意的是，在实际废水中，Lac-beads 的污染物去除效果显著保持，而相应的游离漆酶的效果则严重恶化。
