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用于鉴定宫颈和尿道标本中沙眼衣原体的直接免疫荧光染色法与酶联免疫吸附测定法的比较。

Comparison of direct immunofluorescent staining and an enzyme-linked immunoabsorbent assay for identifying Chlamydia trachomatis in cervical and urethral specimens.

作者信息

Wiesmeier E, Bruckner D A, Malotte C K, Downie A

机构信息

Department of Obstetrics and Gynecology, School of Medicine, University of California, Los Angeles 90024-1703.

出版信息

J Reprod Med. 1989 Aug;34(8):517-9.

PMID:2681749
Abstract

A direct fluorescent antibody (DFA) stain (MicroTrak, Syva) and an enzyme-linked immunoabsorbent assay (Chlamydiazyme, Abbott) were used to detect chlamydial infection in 179 cervical specimens and 56 specimens obtained from the female urethra. Chlamydiazyme, when compared with the DFA technique, revealed a high degree of correlation in these specimens (22 and 21 positive cervical specimens, respectively, and 7 each positive urethral specimens). Since both techniques are readily available, inexpensive and easy to perform, they should become valuable tools in the objective assessment of women at risk of this common and important sexually transmitted disease.

摘要

采用直接荧光抗体(DFA)染色法(MicroTrak,Syva公司)和酶联免疫吸附测定法(Chlamydiazyme,雅培公司)对179份宫颈标本和56份取自女性尿道的标本进行衣原体感染检测。与DFA技术相比,Chlamydiazyme在这些标本中显示出高度相关性(宫颈标本分别有22份和21份呈阳性,尿道标本各有7份呈阳性)。由于这两种技术都容易获得、价格低廉且易于操作,它们应成为客观评估有感染这种常见且重要性传播疾病风险的女性的有价值工具。

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Comparison of direct immunofluorescent staining and an enzyme-linked immunoabsorbent assay for identifying Chlamydia trachomatis in cervical and urethral specimens.用于鉴定宫颈和尿道标本中沙眼衣原体的直接免疫荧光染色法与酶联免疫吸附测定法的比较。
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