Qu Lin, She Pengfei, Wang Yangxia, Liu Fengxia, Zhang Di, Chen Lihua, Luo Zhen, Xu Huan, Qi Yong, Wu Yong
Department of Medicine Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, 410013, China.
Xiangya School of Medicine, Central South University, Changsha, 410013, China.
Microbiologyopen. 2016 Jun;5(3):402-12. doi: 10.1002/mbo3.338. Epub 2016 Jan 27.
Biofilms are defined as aggregation of single cell microorganisms and associated with over 80% of all the microbial infections. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen capable of leading to various infections in immunocompromised people. Recent studies showed that norspermidine, a kind of polyamine, prevented and disrupted biofilm formation by some Gram-negative bacterium. In this study, the effects of norspermidine on P. aeruginosa biofilm formation and eradication were tested. Microtiter plate combined with crystal violet staining was used to study the effects of norspermidine on P. aeruginosa initial attachment, then we employed SEM (scanning electron microscope), qRT-PCR, and QS-related virulence factor assays to investigate how norspermidine prevent biofilm formation by P. aeruginosa. We reported that high-dose norspermidine had bactericide effect on P. aeruginosa, and norspermidine began to inhibit biofilm formation and eradicate 24-h mature biofilm at concentration of 0.1 and 1 mmol/L, respectively, probably by preventing cell-surface attachment, inhibiting swimming motility, and downregulating QS-related genes expression. To investigate the potential utility of norspermidine in preventing device-related infections, we found that catheters immersed with norspermidine were effective in eradicating mature biofilm. These results suggest that norspermidine could be a potent antibiofilm agent for formulating strategies against P. aeruginosa biofilm.
生物膜被定义为单细胞微生物的聚集体，与超过80%的所有微生物感染相关。铜绿假单胞菌是一种革兰氏阴性机会致病菌，能够在免疫功能低下的人群中引发各种感染。最近的研究表明，一种多胺——亚精胺，可预防和破坏一些革兰氏阴性细菌的生物膜形成。在本研究中，测试了亚精胺对铜绿假单胞菌生物膜形成和消除的影响。采用微量滴定板结合结晶紫染色来研究亚精胺对铜绿假单胞菌初始黏附的影响，然后我们利用扫描电子显微镜（SEM）、定量逆转录聚合酶链反应（qRT-PCR）和与群体感应相关的毒力因子测定，来研究亚精胺如何预防铜绿假单胞菌生物膜的形成。我们报告称，高剂量亚精胺对铜绿假单胞菌有杀菌作用，亚精胺分别在浓度为0.1和1 mmol/L时开始抑制生物膜形成并消除24小时成熟生物膜，可能是通过阻止细胞表面黏附、抑制游动性以及下调与群体感应相关的基因表达来实现的。为了研究亚精胺在预防与器械相关感染方面的潜在效用，我们发现浸泡有亚精胺的导管在消除成熟生物膜方面是有效的。这些结果表明，亚精胺可能是一种有效的抗生物膜剂，可用于制定针对铜绿假单胞菌生物膜的策略。
