Mukaida H, Hirai T, Toi M, Nakamura T, Yamashita Y, Kawano K, Toge T, Niimoto M, Hattori T
Dept. of Surgery, Hiroshima Univ.
Gan No Rinsho. 1989 Oct;35(12):1399-406.
The level of epidermal growth factor receptor (EGF-R) has been measured by a competitive binding assay in 45 human esophageal cancer tissues. The EGF-R level in the esophageal cancer tissue was found to be significantly higher than in specimens of gastric or colonic cancer. No correlation was observed, however between the EGF-R level and the pathological findings, such as the depth of the tumoral invasion, the degree of lymph node metastasis, and the histologic staging. Even so, the prognosis of the patients with a high level of EGF-R of more than 50 fmol/mg of protein was significantly worse than those with a low level of EGF-R and less than 50 fmol/mg of protein (p less than 0.01). Further, according to an immunohistochemical assay using anti EGF-R monoclonal antibody, EGF-R was focally stained in the basal cells and in the parabasal cells of normal esophageal mucossa, but was diffuse in almost all tumor cells of ane esophageal cancer. Thus, a good relationship with the quantitative level of EGF-R and its immunoreactivities was demonstrated, indications that EGF-R plays an important role in tumor progression and appears to be a useful prognostic factor in cases of esophageal cancer.
通过竞争性结合试验检测了45例人食管癌组织中表皮生长因子受体（EGF-R）的水平。发现食管癌组织中的EGF-R水平显著高于胃癌或结肠癌标本。然而，未观察到EGF-R水平与病理结果之间的相关性，如肿瘤浸润深度、淋巴结转移程度和组织学分期。即便如此，EGF-R水平高于50 fmol/mg蛋白质的患者的预后明显比EGF-R水平低于50 fmol/mg蛋白质的患者差（p<0.01）。此外，根据使用抗EGF-R单克隆抗体的免疫组织化学检测，EGF-R在正常食管黏膜的基底细胞和副基底细胞中呈局灶性染色，但在食管癌的几乎所有肿瘤细胞中呈弥漫性染色。因此，证明了EGF-R的定量水平与其免疫反应性之间有良好的关系，表明EGF-R在肿瘤进展中起重要作用，并且似乎是食管癌病例中一个有用的预后因素。
