Hou Mingming, Huang Rui, Song Yanni, Feng Di, Jiang Yang, Liu Ming
Department of Orthopedics, The Fourth Affiliated Hospital of Harbin Medical University, Harbin.
Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin.
Jpn J Clin Oncol. 2016 Mar;46(3):222-7. doi: 10.1093/jjco/hyv195. Epub 2016 Jan 26.
ATPase family AAA domain-containing 2 plays an important role in tumor progression including cell cycle, proliferation, apoptosis and chemoresistance. However, the expression of ATPase family AAA domain-containing 2 in colorectal cancer and its significance are still unclear. The aim of this study was to examine the expression of ATPase family AAA domain-containing 2 in colorectal cancer.
Immunohistochemistry was used to determine the expression of ATPase family AAA domain-containing 2 in 155 colorectal cancer and 30 matched adjacent noncancerous tissues. The correlation of ATPase family AAA domain-containing 2 expression with clinicopathological variables was assessed using chi-square test. Patient survival was analyzed using the Kaplan-Meier and log-rank tests. Cox regression was performed for the multivariate analysis of prognostic factors.
High expression of ATPase family AAA domain-containing 2 was detected in 58.1% of the colorectal cancers and was significantly associated with advanced tumor-node-metastasis stage (P = 0.044), poor differentiation (P = 0.028), deep infiltration (P < 0.001), lymphovascular invasion (P = 0.006), lymph node metastasis (P = 0.024) and recurrence (P = 0.022). Patients with high ATPase family AAA domain-containing 2 expression had significantly poorer overall survival and disease-free survival (both P < 0.001) when compared with patients with low expression of ATPase family AAA domain-containing 2. The multivariate analysis showed that ATPase family AAA domain-containing 2 was an independent factor for both overall survival (P = 0.003; hazard ratio (HR): 2.356; 95% confidence interval (CI): 1.335-4.158) and disease-free survival (P = 0.001; HR: 2.643; 95% CI: 1.489-4.693).
These results showed that ATPase family AAA domain-containing 2 overexpression was associated with progression and prognosis of colorectal cancer.
含AAA结构域的ATP酶家族成员2（ATPase family AAA domain-containing 2）在肿瘤进展过程中发挥重要作用，包括细胞周期、增殖、凋亡及化疗耐药性等方面。然而，含AAA结构域的ATP酶家族成员2在结直肠癌中的表达情况及其意义仍不明确。本研究旨在检测含AAA结构域的ATP酶家族成员2在结直肠癌中的表达。
采用免疫组织化学法检测155例结直肠癌组织及30例配对的癌旁非癌组织中含AAA结构域的ATP酶家族成员2的表达。采用卡方检验评估含AAA结构域的ATP酶家族成员2表达与临床病理变量的相关性。使用Kaplan-Meier法和对数秩检验分析患者生存率。进行Cox回归分析以评估预后因素的多变量分析。
58.1%的结直肠癌组织中检测到含AAA结构域的ATP酶家族成员2高表达，且与肿瘤-淋巴结-转移（TNM）分期较晚（P = 0.044）、低分化（P = 0.028）、浸润较深（P < 0.001）、淋巴管侵犯（P = 0.006）、淋巴结转移（P = 0.024）及复发（P = 0.022）显著相关。与含AAA结构域的ATP酶家族成员2低表达的患者相比，高表达患者的总生存期和无病生存期均显著较差（均P < 0.001）。多变量分析显示，含AAA结构域的ATP酶家族成员2是总生存期（P = 0.003；风险比（HR）：2.356；95%置信区间（CI）：1.335 - 4.158）和无病生存期（P = 0.001；HR：2.643；95% CI：1.489 - 4.693）的独立影响因素。
这些结果表明，含AAA结构域的ATP酶家族成员2过表达与结直肠癌的进展及预后相关。
