Qiao Zhu, Li Chun-Long, Zhang Wei
Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, 250100, China.
Plant Mol Biol. 2016 May;91(1-2):53-65. doi: 10.1007/s11103-016-0441-3. Epub 2016 Jan 28.
A key response of plants to moisture stress is stomatal closure, a process mediated by the phytohormone abscisic acid (ABA). Closure is affected by changes in the turgor of the stomatal guard cell. The transcription factor WRKY1 is a part of the regulatory machinery underlying stomatal movements, and through this, in the plant's response to drought stress. The loss-of-function T-DNA insertion mutant wrky1 was particularly sensitive to ABA, with respect to both ion channel regulation and stomatal movements, and less sensitive to drought than the wild type. Complementation of the wrky1 mutant resulted in the recovery of the wild type phenotype. The WRKY1 product localized to the nucleus, and was shown able to bind to the W-box domain in the promoters of MYB2, ABCG40, DREB1A and ABI5, and thereby to control their transcription in response to drought stress or ABA treatment. WRKY1 is thought to act as a negative regulator in guard cell ABA signalling.
植物对水分胁迫的一个关键反应是气孔关闭，这是一个由植物激素脱落酸（ABA）介导的过程。气孔关闭受气孔保卫细胞膨压变化的影响。转录因子WRKY1是气孔运动调控机制的一部分，并通过这一机制参与植物对干旱胁迫的响应。功能缺失的T-DNA插入突变体wrky1在离子通道调节和气孔运动方面对ABA特别敏感，且对干旱的敏感性低于野生型。wrky1突变体的互补导致野生型表型的恢复。WRKY1产物定位于细胞核，并显示能够结合MYB2、ABCG40、DREB1A和ABI5启动子中的W-box结构域，从而在干旱胁迫或ABA处理下控制它们的转录。WRKY1被认为在保卫细胞ABA信号传导中起负调节作用。
