Wu Yanqi, Dobermann Darja, Beale Michael H, Ward Jane L
a Department of Plant Biology and Crop Science , Rothamsted Research , Harpenden , UK.
Nat Prod Res. 2016 Aug;30(15):1731-9. doi: 10.1080/14786419.2015.1137571. Epub 2016 Jan 28.
Ultra high-performance liquid chromatography-mass spectrometry (UHPLC-MS) profiling of a polar solvent extract of juvenile stem tissue of Salix acutifolia Willd. identified a range of phenolic metabolites. Salicortin, 1, a well-known salicinoid, was the major compound present and the study identified young stem tissue of this species as a potential source of this compound for future studies. Three further known metabolites (salicin 2, catechin 3 and tremuloidin 4) were also present. The UHPLC-MS analysis also revealed the presence of a further, less polar, unknown compound, which was isolated via HPLC peak collection. The structure was elucidated by high-resolution mass spectroscopic analysis, 1- and 2-D NMR analysis and chemical derivatisation and was shown to be a novel benzoic acid glycoside 5, which we have named as acutifoliside.
对尖叶柳（Salix acutifolia Willd.）幼茎组织的极性溶剂提取物进行超高效液相色谱-质谱联用（UHPLC-MS）分析，鉴定出一系列酚类代谢产物。著名的水杨素类化合物水杨苷（1）是主要成分，该研究确定该物种的幼茎组织是未来研究中该化合物的潜在来源。另外还存在三种已知代谢产物（水杨苷2、儿茶素3和震颤素4）。UHPLC-MS分析还揭示存在另一种极性较小的未知化合物，通过HPLC峰收集将其分离。通过高分辨率质谱分析、一维和二维核磁共振分析以及化学衍生化阐明了其结构，结果表明它是一种新型苯甲酸糖苷5，我们将其命名为尖叶柳苷。
